A Sanger/pyrosequencing hybrid approach for the generation of high-quality draft assemblies of marine microbial genomes.

@article{citeulike:761828, abstract = {Since its introduction a decade ago, whole-genome shotgun sequencing (WGS) has been the main approach for producing cost-effective and high-quality genome sequence data. Until now, the Sanger sequencing technology that has served as a platform for WGS has not been truly challenged by emerging technologies. The recent introduction of the pyrosequencing-based 454 sequencing platform (454 Life Sciences, Branford, CT) offers a very promising sequencing technology alternative for incorporation in WGS. In this study, we evaluated the utility and cost-effectiveness of a hybrid sequencing approach using 3730xl Sanger data and 454 data to generate higher-quality lower-cost assemblies of microbial genomes compared to current Sanger sequencing strategies alone.}, address = {J. Craig Venter Institute, 9704 Medical Center Drive, Rockville, MD 20850; Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850; Scripps Institution of Oceanography, University of California at San Diego, La Jolla, CA 92093-0202.}, author = {Goldberg, Susanne M D M. and Johnson, Justin and Busam, Dana and Feldblyum, Tamara and Ferriera, Steve and Friedman, Robert and Halpern, Aaron and Khouri, Hoda and Kravitz, Saul A A. and Lauro, Federico M M. and Li, Kelvin and Rogers, Yu-Hui H. and Strausberg, Robert and Sutton, Granger and Tallon, Luke and Thomas, Torsten and Venter, Eli and Frazier, Marvin and Venter, J Craig C. }, citeulike-article-id = {761828}, doi = {http://dx.doi.org/10.1073/pnas.0604351103}, issn = {0027-8424}, journal = {Proc Natl Acad Sci U S A}, keywords = {marine, metagenomics, methods, microbiology, sequencing}, month = {July}, posted-at = {2008-01-11 19:17:25}, priority = {2}, title = {A Sanger/pyrosequencing hybrid approach for the generation of high-quality draft assemblies of marine microbial genomes.}, url = {http://dx.doi.org/10.1073/pnas.0604351103}, year = {2006} }

TY - JOUR ID - citeulike:761828 L3 - citeulike-article-id:761828 TI - A Sanger/pyrosequencing hybrid approach for the generation of high-quality draft assemblies of marine microbial genomes. JF - Proc Natl Acad Sci U S A AD - J. Craig Venter Institute, 9704 Medical Center Drive, Rockville, MD 20850; Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850; Scripps Institution of Oceanography, University of California at San Diego, La Jolla, CA 92093-0202. SN - 0027-8424 N2 - Since its introduction a decade ago, whole-genome shotgun sequencing (WGS) has been the main approach for producing cost-effective and high-quality genome sequence data. Until now, the Sanger sequencing technology that has served as a platform for WGS has not been truly challenged by emerging technologies. The recent introduction of the pyrosequencing-based 454 sequencing platform (454 Life Sciences, Branford, CT) offers a very promising sequencing technology alternative for incorporation in WGS. In this study, we evaluated the utility and cost-effectiveness of a hybrid sequencing approach using 3730xl Sanger data and 454 data to generate higher-quality lower-cost assemblies of microbial genomes compared to current Sanger sequencing strategies alone. KW - marine KW - metagenomics KW - methods KW - microbiology KW - sequencing AU - Goldberg, Susanne M D AU - Johnson, Justin AU - Busam, Dana AU - Feldblyum, Tamara AU - Ferriera, Steve AU - Friedman, Robert AU - Halpern, Aaron AU - Khouri, Hoda AU - Kravitz, Saul A AU - Lauro, Federico M AU - Li, Kelvin AU - Rogers, Yu-Hui AU - Strausberg, Robert AU - Sutton, Granger AU - Tallon, Luke AU - Thomas, Torsten AU - Venter, Eli AU - Frazier, Marvin AU - Venter, J Craig PY - 2006/07/13/ UR - http://dx.doi.org/10.1073/pnas.0604351103 ER -