Dark matter in the genome: evidence of widespread transcription detected by microarray tiling experiments.

@article{citeulike:94619, abstract = {Microarrays provide the opportunity to measure transcription from regions of the genome without bias towards the location of known genes. This technology thus offers an important source of genomic sequence annotation that is complementary to cDNA sequencing and computational gene-finding methods. Recent 'tiling' microarray experiments that assay transcription at regular intervals throughout the genome have shown evidence of large amounts of transcription outside the boundaries of known genes. This transcription is observed in polyadenylated RNA samples and appears to be derived from intergenic regions, from introns of known genes and from sequences antisense to known transcripts. In this article, we discuss different explanations for this phenomenon.}, address = {Rosetta Inpharmatics LLCRosetta Inpharmatics LLC is a wholly owned subsidiary of Merck \& Co., Inc., 401 Terry Avenue North, Seattle, WA 98109, USA.}, author = {Johnson, J. M. and Edwards, S. and Shoemaker, D. and Schadt, E. E. }, citeulike-article-id = {94619}, doi = {http://dx.doi.org/10.1016/j.tig.2004.12.009}, issn = {0168-9525}, journal = {Trends Genet}, keywords = {antisense, dark, dna, genome, hybridization, intergenic, junk, matter, micro-array, region, review, tiling, transcription, transcriptome}, month = {February}, number = {2}, pages = {93--102}, posted-at = {2007-07-06 19:10:13}, priority = {4}, title = {Dark matter in the genome: evidence of widespread transcription detected by microarray tiling experiments.}, url = {http://dx.doi.org/10.1016/j.tig.2004.12.009}, volume = {21}, year = {2005} }

TY - JOUR ID - citeulike:94619 L3 - citeulike-article-id:94619 N2 - Microarrays provide the opportunity to measure transcription from regions of the genome without bias towards the location of known genes. This technology thus offers an important source of genomic sequence annotation that is complementary to cDNA sequencing and computational gene-finding methods. Recent 'tiling' microarray experiments that assay transcription at regular intervals throughout the genome have shown evidence of large amounts of transcription outside the boundaries of known genes. This transcription is observed in polyadenylated RNA samples and appears to be derived from intergenic regions, from introns of known genes and from sequences antisense to known transcripts. In this article, we discuss different explanations for this phenomenon. IS - 2 JF - Trends Genet SN - 0168-9525 AD - Rosetta Inpharmatics LLCRosetta Inpharmatics LLC is a wholly owned subsidiary of Merck & Co., Inc., 401 Terry Avenue North, Seattle, WA 98109, USA. EP - 102 TI - Dark matter in the genome: evidence of widespread transcription detected by microarray tiling experiments. VL - 21 SP - 93 KW - antisense KW - dark KW - dna KW - genome KW - hybridization KW - intergenic KW - junk KW - matter KW - micro-array KW - region KW - review KW - tiling KW - transcription KW - transcriptome AU - Johnson, JM AU - Edwards, S AU - Shoemaker, D AU - Schadt, EE PY - 2005/02// UR - http://dx.doi.org/10.1016/j.tig.2004.12.009 ER -