CDC2L5, a Cdk-like kinase with RS domain, interacts with the ASF/SF2-associated protein p32 and affects splicing in vivo.

@article{citeulike:688555, abstract = {The human CDC2L5 gene encodes a protein of unknown physiological function. This protein is closely related to the cyclin-dependent kinase (Cdks) family and contains an arginine/serine-rich (RS) domain. The Cdks were first identified as crucial regulators of cell-cycle progression, more recently they were found to be involved in transcription and mRNA processing. RS domains are mainly present in proteins regulating pre-mRNA splicing, suggesting CDC2L5 having a possible role in this process. In this study, we demonstrate that CDC2L5 is located in the nucleoplasm, at a higher concentration in speckles, the storage sites for splicing factors. Furthermore, this localization is dependent on the presence of the N-terminal sequence including the RS domain. Then, we report that CDC2L5 directly interacts with the ASF/SF2-associated protein p32, a protein involved in splicing regulation. Overexpression of CDC2L5 constructs disturbs constitutive splicing and switches alternative splice site selection in vivo. These results argue in favor of a functional role of the CDC2L5 kinase in splicing regulation. (c) 2006 Wiley-Liss, Inc.}, address = {Laboratoire Arago, CNRS‐UMR 7628/Universit\'{e} Pierre et Marie Curie, BP 44, F‐66651 Banyuls‐sur‐Mer cedex, France.}, author = {Even, Yasmine and Durieux, Sandrine and Escande, Marie-Line L. and Lozano, Jean Claude C. and Peaucellier, G\'{e}rard and Weil, Dominique and Genevi\`{e}re, Anne-Marie M. }, citeulike-article-id = {688555}, comment = {The name of the main author is missing!}, doi = {http://dx.doi.org/10.1002/jcb.20986}, issn = {0730-2312}, journal = {J Cell Biochem}, keywords = {asf2sf2-associated, cdc2l5, cdk, cdk-like, p32, protein, rs-domain, splicing}, month = {May}, posted-at = {2006-06-07 15:13:23}, priority = {0}, title = {CDC2L5, a Cdk-like kinase with RS domain, interacts with the ASF/SF2-associated protein p32 and affects splicing in vivo.}, url = {http://dx.doi.org/10.1002/jcb.20986}, year = {2006} }

TY - JOUR ID - citeulike:688555 L3 - citeulike-article-id:688555 N2 - The human CDC2L5 gene encodes a protein of unknown physiological function. This protein is closely related to the cyclin-dependent kinase (Cdks) family and contains an arginine/serine-rich (RS) domain. The Cdks were first identified as crucial regulators of cell-cycle progression, more recently they were found to be involved in transcription and mRNA processing. RS domains are mainly present in proteins regulating pre-mRNA splicing, suggesting CDC2L5 having a possible role in this process. In this study, we demonstrate that CDC2L5 is located in the nucleoplasm, at a higher concentration in speckles, the storage sites for splicing factors. Furthermore, this localization is dependent on the presence of the N-terminal sequence including the RS domain. Then, we report that CDC2L5 directly interacts with the ASF/SF2-associated protein p32, a protein involved in splicing regulation. Overexpression of CDC2L5 constructs disturbs constitutive splicing and switches alternative splice site selection in vivo. These results argue in favor of a functional role of the CDC2L5 kinase in splicing regulation. (c) 2006 Wiley-Liss, Inc. JF - J Cell Biochem SN - 0730-2312 AD - Laboratoire Arago, CNRS‐UMR 7628/Université Pierre et Marie Curie, BP 44, F‐66651 Banyuls‐sur‐Mer cedex, France. TI - CDC2L5, a Cdk-like kinase with RS domain, interacts with the ASF/SF2-associated protein p32 and affects splicing in vivo. KW - asf2sf2-associated KW - cdc2l5 KW - cdk KW - cdk-like KW - p32 KW - protein KW - rs-domain KW - splicing N1 - The name of the main author is missing! AU - Even, Yasmine AU - Durieux, Sandrine AU - Escande, Marie-Line AU - Lozano, Jean Claude AU - Peaucellier, Gérard AU - Weil, Dominique AU - Genevière, Anne-Marie PY - 2006/05/23/ UR - http://dx.doi.org/10.1002/jcb.20986 ER -