A two-entropies analysis to identify functional positions in the transmembrane region of class A G protein-coupled receptors.

@article{citeulike:770915, abstract = {Residues in the transmembrane region of G protein-coupled receptors (GPCRs) are important for ligand binding and activation, but the function of individual positions is poorly understood. Using a sequence alignment of class A GPCRs (grouped in subfamilies), we propose a so-called "two-entropies analysis" to determine the potential role of individual positions in the transmembrane region of class A GPCRs. In our approach, such positions appear scattered, while largely clustered according to their biological function. Our method appears superior when compared to other bioinformatics approaches, such as the evolutionary trace method, entropy-variability plot, and correlated mutation analysis, both qualitatively and quantitatively.}, address = {Division of Medicinal Chemistry, Leiden/Amsterdam Center for Drug Research, Leiden University, Leiden, The Netherlands.}, author = {Ye, K. and Lameijer, E. W. and Beukers, M. W. and Ijzerman, A. P. }, citeulike-article-id = {770915}, doi = {http://dx.doi.org/10.1002/prot.20899}, issn = {1097-0134}, journal = {Proteins}, keywords = {activation, gpcr, sequence, transmembrane}, month = {June}, number = {4}, pages = {1018--1030}, posted-at = {2006-07-24 10:21:33}, priority = {4}, title = {A two-entropies analysis to identify functional positions in the transmembrane region of class A G protein-coupled receptors.}, url = {http://dx.doi.org/10.1002/prot.20899}, volume = {63}, year = {2006} }

TY - JOUR ID - citeulike:770915 L3 - citeulike-article-id:770915 N2 - Residues in the transmembrane region of G protein-coupled receptors (GPCRs) are important for ligand binding and activation, but the function of individual positions is poorly understood. Using a sequence alignment of class A GPCRs (grouped in subfamilies), we propose a so-called "two-entropies analysis" to determine the potential role of individual positions in the transmembrane region of class A GPCRs. In our approach, such positions appear scattered, while largely clustered according to their biological function. Our method appears superior when compared to other bioinformatics approaches, such as the evolutionary trace method, entropy-variability plot, and correlated mutation analysis, both qualitatively and quantitatively. IS - 4 JF - Proteins SN - 1097-0134 AD - Division of Medicinal Chemistry, Leiden/Amsterdam Center for Drug Research, Leiden University, Leiden, The Netherlands. EP - 1030 TI - A two-entropies analysis to identify functional positions in the transmembrane region of class A G protein-coupled receptors. VL - 63 SP - 1018 KW - activation KW - gpcr KW - sequence KW - transmembrane AU - Ye, K AU - Lameijer, EW AU - Beukers, MW AU - Ijzerman, AP PY - 2006/06/01/ UR - http://dx.doi.org/10.1002/prot.20899 ER -