Imaging and characterizing influenza A virus mRNA transport in living cells

@article{citeulike:3157981, abstract = {The mechanisms of influenza A virus mRNA intracellular transport are still not clearly understood. Here, we visualized the distribution and transport of influenza A virus mRNA in living cells using molecular beacon (MB) technology. Confocal-FRAP measurements determined that the transport of influenza A virus intronless mRNA, in both nucleus and cytoplasm, was energy dependent, being similar to that of Poly(A)+ RNA. Drug inhibition studies in living cells revealed that the export of influenza A virus mRNA is independent of the CRM1 pathway, while the function of RNA polymerase II (RNAP-II) may be needed. In addition, viral NS1 protein and cellular TAP protein were found associated with influenza A virus mRNA in the cell nucleus. These findings characterize influenza A virus mRNA transport in living cells and suggest that influenza A virus mRNA may be exported from the nucleus by the cellular TAP/p15 pathway with NS1 protein and RNAP-II participation. 10.1093/nar/gkn475}, author = {Wang, Wei and Cui, Zong-Qiang and Han, Han and Zhang, Zhi-Ping and Wei, Hong-Ping and Zhou, Ya-Feng and Chen, Ze and Zhang, Xian-En }, citeulike-article-id = {3157981}, doi = {http://dx.doi.org/10.1093/nar/gkn475}, journal = {Nucl. Acids Res.}, keywords = {influenza, replication}, month = {September}, number = {15}, pages = {4913--4928}, posted-at = {2008-08-27 09:56:26}, priority = {2}, title = {Imaging and characterizing influenza A virus mRNA transport in living cells}, url = {http://dx.doi.org/10.1093/nar/gkn475}, volume = {36}, year = {2008} }

TY - JOUR ID - citeulike:3157981 L3 - citeulike-article-id:3157981 N2 - The mechanisms of influenza A virus mRNA intracellular transport are still not clearly understood. Here, we visualized the distribution and transport of influenza A virus mRNA in living cells using molecular beacon (MB) technology. Confocal-FRAP measurements determined that the transport of influenza A virus intronless mRNA, in both nucleus and cytoplasm, was energy dependent, being similar to that of Poly(A)+ RNA. Drug inhibition studies in living cells revealed that the export of influenza A virus mRNA is independent of the CRM1 pathway, while the function of RNA polymerase II (RNAP-II) may be needed. In addition, viral NS1 protein and cellular TAP protein were found associated with influenza A virus mRNA in the cell nucleus. These findings characterize influenza A virus mRNA transport in living cells and suggest that influenza A virus mRNA may be exported from the nucleus by the cellular TAP/p15 pathway with NS1 protein and RNAP-II participation. 10.1093/nar/gkn475 IS - 15 JF - Nucl. Acids Res. EP - 4928 TI - Imaging and characterizing influenza A virus mRNA transport in living cells VL - 36 SP - 4913 KW - influenza KW - replication AU - Wang, Wei AU - Cui, Zong-Qiang AU - Han, Han AU - Zhang, Zhi-Ping AU - Wei, Hong-Ping AU - Zhou, Ya-Feng AU - Chen, Ze AU - Zhang, Xian-En PY - 2008/09/01/ UR - http://dx.doi.org/10.1093/nar/gkn475 ER -