The effect of particle design on cellular internalization pathways

@article{citeulike:3141910, abstract = {10.1073/pnas.0801763105 The interaction of particles with cells is known to be strongly influenced by particle size, but little is known about the interdependent role that size, shape, and surface chemistry have on cellular internalization and intracellular trafficking. We report on the internalization of specially designed, monodisperse hydrogel particles into HeLa cells as a function of size, shape, and surface charge. We employ a top-down particle fabrication technique called PRINT that is able to generate uniform populations of organic micro- and nanoparticles with complete control of size, shape, and surface chemistry. Evidence of particle internalization was obtained by using conventional biological techniques and transmission electron microscopy. These findings suggest that HeLa cells readily internalize nonspherical particles with dimensions as large as 3 μm by using several different mechanisms of endocytosis. Moreover, it was found that rod-like particles enjoy an appreciable advantage when it comes to internalization rates, reminiscent of the advantage that many rod-like bacteria have for internalization in nonphagocytic cells.}, author = {Gratton, Stephanie E. and Ropp, Patricia A. and Pohlhaus, Patrick D. and Luft, Christopher J. and Madden, Victoria J. and Napier, Mary E. and Desimone, Joseph M. }, citeulike-article-id = {3141910}, doi = {http://dx.doi.org/10.1073/pnas.0801763105}, journal = {Proceedings of the National Academy of Sciences}, keywords = {entry, macrophage, raft}, number = {33}, pages = {11613--11618}, posted-at = {2008-08-21 15:24:26}, priority = {2}, title = {The effect of particle design on cellular internalization pathways}, url = {http://dx.doi.org/10.1073/pnas.0801763105}, volume = {105}, year = {2008} }

TY - JOUR ID - citeulike:3141910 L3 - citeulike-article-id:3141910 N2 - 10.1073/pnas.0801763105 The interaction of particles with cells is known to be strongly influenced by particle size, but little is known about the interdependent role that size, shape, and surface chemistry have on cellular internalization and intracellular trafficking. We report on the internalization of specially designed, monodisperse hydrogel particles into HeLa cells as a function of size, shape, and surface charge. We employ a top-down particle fabrication technique called PRINT that is able to generate uniform populations of organic micro- and nanoparticles with complete control of size, shape, and surface chemistry. Evidence of particle internalization was obtained by using conventional biological techniques and transmission electron microscopy. These findings suggest that HeLa cells readily internalize nonspherical particles with dimensions as large as 3 μm by using several different mechanisms of endocytosis. Moreover, it was found that rod-like particles enjoy an appreciable advantage when it comes to internalization rates, reminiscent of the advantage that many rod-like bacteria have for internalization in nonphagocytic cells. IS - 33 JF - Proceedings of the National Academy of Sciences EP - 11618 TI - The effect of particle design on cellular internalization pathways VL - 105 SP - 11613 KW - entry KW - macrophage KW - raft AU - Gratton, Stephanie AU - Ropp, Patricia AU - Pohlhaus, Patrick AU - Luft, Christopher AU - Madden, Victoria AU - Napier, Mary AU - Desimone, Joseph PY - 2008//19/ UR - http://dx.doi.org/10.1073/pnas.0801763105 ER -