Endocytic pathway followed by dengue virus to infect the mosquito cell line C6/36 HT

@article{citeulike:3134002, abstract = {The endocytic pathway followed by dengue virus to infect the mosquito cells C6/36 HT was analyzed. Using DIL-labeled virions and real-time imaging it was determined that viral entry into C6/36 HT takes approximately 5 to 7 min. Pretreatment of C6/36 HT cells with sucrose and bafilomycin A, but not filipin, inhibited dengue virus infection up to 80\%. Furthermore, the overexpression of dominant-negative mutants of Eps15, a molecule required for the formation of clathrin-coated vesicles, reduced dengue infection up to 50\%, indicating that dengue virus entry is through clathrin-mediated endocytosis and is pH-dependent. By double-immunofluorescence assays, DIL-labeled particles were colocalized with early endosomes at 5 min and with lysosomes mainly at 30 min post-infection. Finally, disruption of the microtubule and microfilaments by nocodazole and by cytochalasin D reduced viral infection by more than 80\%. Taken together these results indicate that dengue virions enter into C6/36 HT cells by clathrin-mediated endocytosis, using the endosomal pathway from early endosomes to acidic lysosomes before viral RNA is released into the cytoplasm.}, author = {Mosso, Clemente and Galv\'{a}n-Mendoza, Ivan J. and Ludert, Juan E. and Del}, citeulike-article-id = {3134002}, doi = {http://dx.doi.org/10.1016/j.virol.2008.05.012}, journal = {Virology}, keywords = {entry, flavivirus}, month = {August}, number = {1}, pages = {193--199}, posted-at = {2008-08-18 18:15:05}, priority = {2}, title = {Endocytic pathway followed by dengue virus to infect the mosquito cell line C6/36 HT}, url = {http://dx.doi.org/10.1016/j.virol.2008.05.012}, volume = {378}, year = {2008} }

TY - JOUR ID - citeulike:3134002 L3 - citeulike-article-id:3134002 N2 - The endocytic pathway followed by dengue virus to infect the mosquito cells C6/36 HT was analyzed. Using DIL-labeled virions and real-time imaging it was determined that viral entry into C6/36 HT takes approximately 5 to 7 min. Pretreatment of C6/36 HT cells with sucrose and bafilomycin A, but not filipin, inhibited dengue virus infection up to 80%. Furthermore, the overexpression of dominant-negative mutants of Eps15, a molecule required for the formation of clathrin-coated vesicles, reduced dengue infection up to 50%, indicating that dengue virus entry is through clathrin-mediated endocytosis and is pH-dependent. By double-immunofluorescence assays, DIL-labeled particles were colocalized with early endosomes at 5 min and with lysosomes mainly at 30 min post-infection. Finally, disruption of the microtubule and microfilaments by nocodazole and by cytochalasin D reduced viral infection by more than 80%. Taken together these results indicate that dengue virions enter into C6/36 HT cells by clathrin-mediated endocytosis, using the endosomal pathway from early endosomes to acidic lysosomes before viral RNA is released into the cytoplasm. IS - 1 JF - Virology EP - 199 TI - Endocytic pathway followed by dengue virus to infect the mosquito cell line C6/36 HT VL - 378 SP - 193 KW - entry KW - flavivirus AU - Mosso, Clemente AU - Galván-Mendoza, Ivan AU - Ludert, Juan AU - Del PY - 2008/08/15/ UR - http://dx.doi.org/10.1016/j.virol.2008.05.012 ER -