West Nile Virus Entry Requires Cholesterol-Rich Membrane Microdomains and Is Independent of alphavbeta3 Integrin

@article{citeulike:2805573, abstract = {West Nile virus (WNV) has been the leading cause of viral encephalitis in the United States since 1999. The endocytic processes involved in the internalization of infectious WNV by various cell types are not well characterized, and the involvement of cholesterol-rich membrane microdomains, or lipid rafts, in the life cycle of WNV has not been investigated previously. In this study, we found that the depletion of cellular cholesterol levels by brief treatment with methyl--cyclodextrin resulted in a 100-fold reduction of the titers of infectious WNV released into the culture supernatant, as well as a reduction in the number of WNV genome copies in the cholesterol-depleted cells. The addition of exogenous cholesterol to cholesterol-depleted cells reversed this effect. Cholesterol depletion postinfection did not affect WNV growth, suggesting that the effect occurs at the level of WNV entry. We also showed that while WNV entry did not require {alpha}v3 integrin and focal adhesion kinase, WNV particles failed to be internalized by cholesterol-depleted cells. Finally, we showed the colocalization of the WNV envelope protein and cholera toxin B, which is internalized in a lipid raft-dependent pathway, in microdomain clusters at the plasma membrane. These data suggest that WNV utilizes lipid rafts during initial stages of internalization and that the lipid rafts may contain a factor(s) that may enhance WNV endocytosis. 10.1128/JVI.00008-08}, author = {Medigeshi, Guruprasad R. and Hirsch, Alec J. and Streblow, Daniel N. and Nikolich-Zugich, Janko and Nelson, Jay A. }, citeulike-article-id = {2805573}, doi = {http://dx.doi.org/10.1128/JVI.00008-08}, journal = {J. Virol.}, keywords = {entry, flavivirus, raft, receptor}, month = {June}, number = {11}, pages = {5212--5219}, posted-at = {2008-05-16 17:55:46}, priority = {2}, title = {West Nile Virus Entry Requires Cholesterol-Rich Membrane Microdomains and Is Independent of {alpha}v{beta}3 Integrin}, url = {http://dx.doi.org/10.1128/JVI.00008-08}, volume = {82}, year = {2008} }

TY - JOUR ID - citeulike:2805573 L3 - citeulike-article-id:2805573 N2 - West Nile virus (WNV) has been the leading cause of viral encephalitis in the United States since 1999. The endocytic processes involved in the internalization of infectious WNV by various cell types are not well characterized, and the involvement of cholesterol-rich membrane microdomains, or lipid rafts, in the life cycle of WNV has not been investigated previously. In this study, we found that the depletion of cellular cholesterol levels by brief treatment with methyl--cyclodextrin resulted in a 100-fold reduction of the titers of infectious WNV released into the culture supernatant, as well as a reduction in the number of WNV genome copies in the cholesterol-depleted cells. The addition of exogenous cholesterol to cholesterol-depleted cells reversed this effect. Cholesterol depletion postinfection did not affect WNV growth, suggesting that the effect occurs at the level of WNV entry. We also showed that while WNV entry did not require {alpha}v3 integrin and focal adhesion kinase, WNV particles failed to be internalized by cholesterol-depleted cells. Finally, we showed the colocalization of the WNV envelope protein and cholera toxin B, which is internalized in a lipid raft-dependent pathway, in microdomain clusters at the plasma membrane. These data suggest that WNV utilizes lipid rafts during initial stages of internalization and that the lipid rafts may contain a factor(s) that may enhance WNV endocytosis. 10.1128/JVI.00008-08 IS - 11 JF - J. Virol. EP - 5219 TI - West Nile Virus Entry Requires Cholesterol-Rich Membrane Microdomains and Is Independent of {alpha}v{beta}3 Integrin VL - 82 SP - 5212 KW - entry KW - flavivirus KW - raft KW - receptor AU - Medigeshi, Guruprasad AU - Hirsch, Alec AU - Streblow, Daniel AU - Nikolich-Zugich, Janko AU - Nelson, Jay PY - 2008/06/01/ UR - http://dx.doi.org/10.1128/JVI.00008-08 ER -