The HIV-1 Vif Protein Mediates Degradation of Vpr and Reduces Vpr-Induced Cell Cycle Arrest

@article{citeulike:2787447, abstract = {Prior work has implicated viral protein R (Vpr) in the arrest of human immunodeficiency virus type 1 (HIV-1)-infected cells in the G2 phase of the cell cycle, associated with increased viral replication and host cell apoptosis. We and others have recently shown that virion infectivity factor (Vif ) also plays a role in the G2 arrest of HIV-1-infected cells. Here, we demonstrate that, paradoxically, at early time points postinfection, Vif expression blocks Vpr-mediated G2 arrest, while deletion of Vif from the HIV-1 genome leads to a marked increase in G2 arrest of infected CD4 T-cells. Consistent with this increased G2 arrest, T-cells infected with Vif-deleted HIV-1 express higher levels of Vpr protein than cells infected with wild-type virus. Further, expression of exogenous Vif inhibits the expression of Vpr, associated with a decrease in G2 arrest of both infected and transfected cells. Treatment with the proteasome inhibitor MG132 increases Vpr protein expression and G2 arrest in wild-type, but not Vif-deleted, NL4-3-infected cells, and in cells cotransfected with Vif and Vpr. In addition, Vpr coimmunoprecipitates with Vif in cotransfected cells in the presence of MG132. This suggests that inhibition of Vpr by Vif is mediated at least in part by proteasomal degradation, similar to Vif-induced degradation of APOBEC3G. Together, these data show that Vif mediates the degradation of Vpr and modulates Vpr-induced G2 arrest in HIV-1-infected T-cells.}, author = {Wang, Jiangfang and Shackelford, Jason M. and Selliah, Nithianandan and Shivers, Debra K. and O'Neill, Eduardo and Garcia, Victor J. and Muthumani, Karuppiah and Weiner, David and Yu, Xiao-Fang and Gabuzda, Dana and Finkel, Terri H. }, citeulike-article-id = {2787447}, doi = {http://dx.doi.org/10.1089/dna.2007.0707}, journal = {DNA and Cell Biology}, keywords = {hiv, replication, vpr}, number = {5}, pages = {267--277}, posted-at = {2008-09-01 08:42:44}, priority = {2}, title = {The HIV-1 Vif Protein Mediates Degradation of Vpr and Reduces Vpr-Induced Cell Cycle Arrest}, url = {http://dx.doi.org/10.1089/dna.2007.0707}, volume = {27}, year = {2008} }

TY - JOUR ID - citeulike:2787447 L3 - citeulike-article-id:2787447 N2 - Prior work has implicated viral protein R (Vpr) in the arrest of human immunodeficiency virus type 1 (HIV-1)-infected cells in the G2 phase of the cell cycle, associated with increased viral replication and host cell apoptosis. We and others have recently shown that virion infectivity factor (Vif ) also plays a role in the G2 arrest of HIV-1-infected cells. Here, we demonstrate that, paradoxically, at early time points postinfection, Vif expression blocks Vpr-mediated G2 arrest, while deletion of Vif from the HIV-1 genome leads to a marked increase in G2 arrest of infected CD4 T-cells. Consistent with this increased G2 arrest, T-cells infected with Vif-deleted HIV-1 express higher levels of Vpr protein than cells infected with wild-type virus. Further, expression of exogenous Vif inhibits the expression of Vpr, associated with a decrease in G2 arrest of both infected and transfected cells. Treatment with the proteasome inhibitor MG132 increases Vpr protein expression and G2 arrest in wild-type, but not Vif-deleted, NL4-3-infected cells, and in cells cotransfected with Vif and Vpr. In addition, Vpr coimmunoprecipitates with Vif in cotransfected cells in the presence of MG132. This suggests that inhibition of Vpr by Vif is mediated at least in part by proteasomal degradation, similar to Vif-induced degradation of APOBEC3G. Together, these data show that Vif mediates the degradation of Vpr and modulates Vpr-induced G2 arrest in HIV-1-infected T-cells. IS - 5 JF - DNA and Cell Biology EP - 277 TI - The HIV-1 Vif Protein Mediates Degradation of Vpr and Reduces Vpr-Induced Cell Cycle Arrest VL - 27 SP - 267 KW - hiv KW - replication KW - vpr AU - Wang, Jiangfang AU - Shackelford, Jason AU - Selliah, Nithianandan AU - Shivers, Debra AU - O'Neill, Eduardo AU - Garcia, Victor AU - Muthumani, Karuppiah AU - Weiner, David AU - Yu, Xiao-Fang AU - Gabuzda, Dana AU - Finkel, Terri PY - 2008/// UR - http://dx.doi.org/10.1089/dna.2007.0707 ER -