The implications of alternative splicing in the ENCODE protein complement.

@article{citeulike:1203303, abstract = {Alternative premessenger RNA splicing enables genes to generate more than one gene product. Splicing events that occur within protein coding regions have the potential to alter the biological function of the expressed protein and even to create new protein functions. Alternative splicing has been suggested as one explanation for the discrepancy between the number of human genes and functional complexity. Here, we carry out a detailed study of the alternatively spliced gene products annotated in the ENCODE pilot project. We find that alternative splicing in human genes is more frequent than has commonly been suggested, and we demonstrate that many of the potential alternative gene products will have markedly different structure and function from their constitutively spliced counterparts. For the vast majority of these alternative isoforms, little evidence exists to suggest they have a role as functional proteins, and it seems unlikely that the spectrum of conventional enzymatic or structural functions can be substantially extended through alternative splicing.}, address = {Structural Computational Biology Programme, Spanish National Cancer Research Centre, E-28029 Madrid, Spain.}, author = {Tress, M. L. and Martelli, P. L. and Frankish, A. and Reeves, G. A. and Wesselink, J. J. and Yeats, C. and Olason, P. L. and Albrecht, M. and Hegyi, H. and Giorgetti, A. and Raimondo, D. and Lagarde, J. and Laskowski, R. A. and L\'{o}pez, G. and Sadowski, M. I. and Watson, J. D. and Fariselli, P. and Rossi, I. and Nagy, A. and Kai, W. and Størling, Z. and Orsini, M. and Assenov, Y. and Blankenburg, H. and Huthmacher, C. and Ram\'{i}rez, F. and Schlicker, A. and Denoeud, F. and Jones, P. and Kerrien, S. and Orchard, S. and Antonarakis, S. E. and Reymond, A. and Birney, E. and Brunak, S. and Casadio, R. and Guigo, R. and Harrow, J. and Hermjakob, H. and Jones, D. T. and Lengauer, T. and A Orengo, C. and Patthy, L. and Thornton, J. M. and Tramontano, A. and Valencia, A. }, citeulike-article-id = {1203303}, doi = {http://dx.doi.org/10.1073/pnas.0700800104}, issn = {0027-8424}, journal = {Proc Natl Acad Sci U S A}, keywords = {bologna08, splicing, structure}, month = {March}, number = {13}, pages = {5495--5500}, posted-at = {2008-02-18 09:45:18}, priority = {2}, title = {The implications of alternative splicing in the ENCODE protein complement.}, url = {http://dx.doi.org/10.1073/pnas.0700800104}, volume = {104}, year = {2007} }

TY - JOUR ID - citeulike:1203303 L3 - citeulike-article-id:1203303 N2 - Alternative premessenger RNA splicing enables genes to generate more than one gene product. Splicing events that occur within protein coding regions have the potential to alter the biological function of the expressed protein and even to create new protein functions. Alternative splicing has been suggested as one explanation for the discrepancy between the number of human genes and functional complexity. Here, we carry out a detailed study of the alternatively spliced gene products annotated in the ENCODE pilot project. We find that alternative splicing in human genes is more frequent than has commonly been suggested, and we demonstrate that many of the potential alternative gene products will have markedly different structure and function from their constitutively spliced counterparts. For the vast majority of these alternative isoforms, little evidence exists to suggest they have a role as functional proteins, and it seems unlikely that the spectrum of conventional enzymatic or structural functions can be substantially extended through alternative splicing. IS - 13 JF - Proc Natl Acad Sci U S A SN - 0027-8424 AD - Structural Computational Biology Programme, Spanish National Cancer Research Centre, E-28029 Madrid, Spain. EP - 5500 TI - The implications of alternative splicing in the ENCODE protein complement. VL - 104 SP - 5495 KW - bologna08 KW - splicing KW - structure AU - Tress, ML AU - Martelli, PL AU - Frankish, A AU - Reeves, GA AU - Wesselink, JJ AU - Yeats, C AU - Olason, PL AU - Albrecht, M AU - Hegyi, H AU - Giorgetti, A AU - Raimondo, D AU - Lagarde, J AU - Laskowski, RA AU - López, G AU - Sadowski, MI AU - Watson, JD AU - Fariselli, P AU - Rossi, I AU - Nagy, A AU - Kai, W AU - Størling, Z AU - Orsini, M AU - Assenov, Y AU - Blankenburg, H AU - Huthmacher, C AU - Ramírez, F AU - Schlicker, A AU - Denoeud, F AU - Jones, P AU - Kerrien, S AU - Orchard, S AU - Antonarakis, SE AU - Reymond, A AU - Birney, E AU - Brunak, S AU - Casadio, R AU - Guigo, R AU - Harrow, J AU - Hermjakob, H AU - Jones, DT AU - Lengauer, T AU - A Orengo, C AU - Patthy, L AU - Thornton, JM AU - Tramontano, A AU - Valencia, A PY - 2007/03/27/ UR - http://dx.doi.org/10.1073/pnas.0700800104 ER -