Analysis of the spacer DNA between the cyclic AMP receptor protein binding site and the lac promoter.

by: U Flatow, GV Rajendrakumar, S Garges

J Bacteriol, Vol. 178, No. 8. (April 1996), pp. 2436-2439.

View FullText article

Pubmed, Hubmed

Reviews [Write a review of this article]

There are no reviews of this article

Find related articles from these CiteULike users

wilsoros

Find related articles with these CiteULike tags

camp, repression

摘要

The role of the spacer region DNA between the cyclic AMP receptor protein (CRP) site and the RNA polymerase in the lac promoter was examined. We wanted to determine whether the wild-type DNA sequence of this region was an absolute requirement for CRP activation of lac transcription. The sequence of a 9-bp stretch of the spacer, from -41 to -49 relative to the start of transcription, was randomized, and the effect of randomization on lac expression was investigated in vitro and in vivo. We found that the spacer contains no specific sequence determinants for CRP activation of lac transcription; fewer than 1% of the mutants displayed greater than a 50% decrease in CRP activation of lac transcription.

@article{citeulike:903034, abstract = {The role of the spacer region DNA between the cyclic AMP receptor protein (CRP) site and the RNA polymerase in the lac promoter was examined. We wanted to determine whether the wild-type DNA sequence of this region was an absolute requirement for CRP activation of lac transcription. The sequence of a 9-bp stretch of the spacer, from -41 to -49 relative to the start of transcription, was randomized, and the effect of randomization on lac expression was investigated in vitro and in vivo. We found that the spacer contains no specific sequence determinants for CRP activation of lac transcription; fewer than 1\% of the mutants displayed greater than a 50\% decrease in CRP activation of lac transcription.}, address = {Developmental Genetics Section, Laboratory of Molecular Biology, National Cancer Institute, Bethesda, Maryland 20892-4255, USA.}, author = {Flatow, U. and Rajendrakumar, G. V. and Garges, S. }, citeulike-article-id = {903034}, issn = {0021-9193}, journal = {J Bacteriol}, keywords = {camp, repression}, month = {April}, number = {8}, pages = {2436--2439}, posted-at = {2006-10-18 06:22:55}, priority = {2}, title = {Analysis of the spacer DNA between the cyclic AMP receptor protein binding site and the lac promoter.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/8636052}, volume = {178}, year = {1996} }

RIS record

TY - JOUR ID - citeulike:903034 L3 - citeulike-article-id:903034 N2 - The role of the spacer region DNA between the cyclic AMP receptor protein (CRP) site and the RNA polymerase in the lac promoter was examined. We wanted to determine whether the wild-type DNA sequence of this region was an absolute requirement for CRP activation of lac transcription. The sequence of a 9-bp stretch of the spacer, from -41 to -49 relative to the start of transcription, was randomized, and the effect of randomization on lac expression was investigated in vitro and in vivo. We found that the spacer contains no specific sequence determinants for CRP activation of lac transcription; fewer than 1% of the mutants displayed greater than a 50% decrease in CRP activation of lac transcription. IS - 8 JF - J Bacteriol SN - 0021-9193 AD - Developmental Genetics Section, Laboratory of Molecular Biology, National Cancer Institute, Bethesda, Maryland 20892-4255, USA. EP - 2439 TI - Analysis of the spacer DNA between the cyclic AMP receptor protein binding site and the lac promoter. VL - 178 SP - 2436 KW - camp KW - repression AU - Flatow, U AU - Rajendrakumar, GV AU - Garges, S PY - 1996/04// UR - http://view.ncbi.nlm.nih.gov/pubmed/8636052 ER -

RIS BibTeX