Nuclear Envelope Breakdown Proceeds by Microtubule-Induced Tearing of the Lamina

by: Joël Beaudouin, Daniel Gerlich, Nathalie Daigle, Roland Eils, Jan Ellenberg

Cell, Vol. 108, No. 1. (11 January 2002), pp. 83-96.

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摘要

The mechanism of nuclear envelope breakdown (NEBD) was investigated in live cells. Early spindle microtubules caused folds and invaginations in the NE up to one hour prior to NEBD, creating mechanical tension in the nuclear lamina. The first gap in the NE appeared before lamin B depolymerization, at the site of maximal tension, by a tearing mechanism. Gap formation relaxed this tension and dramatically accelerated the rate of chromosome condensation. The hole produced in the NE then rapidly expanded over the nuclear surface. NE fragments remaining on chromosomes were removed toward the centrosomes in a microtubule-dependent manner, suggesting a mechanism mediated by a minus-end-directed motor.

@article{beaudouin2002, abstract = {The mechanism of nuclear envelope breakdown (NEBD) was investigated in live cells. Early spindle microtubules caused folds and invaginations in the NE up to one hour prior to NEBD, creating mechanical tension in the nuclear lamina. The first gap in the NE appeared before lamin B depolymerization, at the site of maximal tension, by a tearing mechanism. Gap formation relaxed this tension and dramatically accelerated the rate of chromosome condensation. The hole produced in the NE then rapidly expanded over the nuclear surface. NE fragments remaining on chromosomes were removed toward the centrosomes in a microtubule-dependent manner, suggesting a mechanism mediated by a minus-end-directed motor.}, author = {Beaudouin, Jo\"{e}l and Gerlich, Daniel and Daigle, Nathalie and Eils, Roland and Ellenberg, Jan }, citeulike-article-id = {2937854}, doi = {http://dx.doi.org/10.1016/S0092-8674(01)00627-4}, journal = {Cell}, keywords = {breakdown, directed, end, envelope, lamin, minus, nuclear}, month = {January}, number = {1}, pages = {83--96}, posted-at = {2008-06-27 19:03:56}, priority = {2}, title = {Nuclear Envelope Breakdown Proceeds by Microtubule-Induced Tearing of the Lamina}, url = {http://dx.doi.org/10.1016/S0092-8674(01)00627-4}, volume = {108}, year = {2002} }

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TY - JOUR ID - beaudouin2002 L3 - citeulike-article-id:2937854 N2 - The mechanism of nuclear envelope breakdown (NEBD) was investigated in live cells. Early spindle microtubules caused folds and invaginations in the NE up to one hour prior to NEBD, creating mechanical tension in the nuclear lamina. The first gap in the NE appeared before lamin B depolymerization, at the site of maximal tension, by a tearing mechanism. Gap formation relaxed this tension and dramatically accelerated the rate of chromosome condensation. The hole produced in the NE then rapidly expanded over the nuclear surface. NE fragments remaining on chromosomes were removed toward the centrosomes in a microtubule-dependent manner, suggesting a mechanism mediated by a minus-end-directed motor. IS - 1 JF - Cell EP - 96 TI - Nuclear Envelope Breakdown Proceeds by Microtubule-Induced Tearing of the Lamina VL - 108 SP - 83 KW - breakdown KW - directed KW - end KW - envelope KW - lamin KW - minus KW - nuclear AU - Beaudouin, Joël AU - Gerlich, Daniel AU - Daigle, Nathalie AU - Eils, Roland AU - Ellenberg, Jan PY - 2002/01/11/ UR - http://dx.doi.org/10.1016/S0092-8674(01)00627-4 ER -

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