A Mitotic Lamin B-Matrix Induced by RanGTP Required for Spindle Assembly

@article{tsai2006, abstract = {Mitotic spindle morphogenesis is a series of highly coordinated movements that lead to chromosome segregation and cytokinesis. We report that the intermediate filament protein lamin B, a component of the interphase nuclear lamina, functions in spindle assembly. Lamin B assembled into a matrix-like network in mitosis through a process that depended on the presence of the guanosine triphosphate (GTP)-bound form of the small GTPase Ran. Depletion of lamin B resulted in defects in spindle assembly. Dominant negative mutant lamin B proteins that disrupt lamin B assembly in interphase nuclei also disrupted spindle assembly in mitosis. Furthermore, lamin B was essential for the formation of the mitotic matrix that tethers a number of spindle assembly factors. We propose that lamin B is a structural component of the long-sought-after spindle matrix that promotes microtubule assembly and organization in mitosis. 10.1126/science.1122771}, author = {Tsai, Ming-Ying and Wang, Shusheng and Heidinger, Jill and Shumaker, Dale and Adam, Stephen A. and Goldman, Robert D. and Zheng, Yixian }, citeulike-article-id = {2857663}, doi = {http://dx.doi.org/10.1126/science.1122771}, journal = {Science}, keywords = {lamin, matrix}, month = {March}, pages = {1122771+}, posted-at = {2008-06-02 19:51:12}, priority = {0}, title = {A Mitotic Lamin B-Matrix Induced by RanGTP Required for Spindle Assembly}, url = {http://dx.doi.org/10.1126/science.1122771}, year = {2006} }

TY - JOUR ID - tsai2006 L3 - citeulike-article-id:2857663 N2 - Mitotic spindle morphogenesis is a series of highly coordinated movements that lead to chromosome segregation and cytokinesis. We report that the intermediate filament protein lamin B, a component of the interphase nuclear lamina, functions in spindle assembly. Lamin B assembled into a matrix-like network in mitosis through a process that depended on the presence of the guanosine triphosphate (GTP)-bound form of the small GTPase Ran. Depletion of lamin B resulted in defects in spindle assembly. Dominant negative mutant lamin B proteins that disrupt lamin B assembly in interphase nuclei also disrupted spindle assembly in mitosis. Furthermore, lamin B was essential for the formation of the mitotic matrix that tethers a number of spindle assembly factors. We propose that lamin B is a structural component of the long-sought-after spindle matrix that promotes microtubule assembly and organization in mitosis. 10.1126/science.1122771 JF - Science TI - A Mitotic Lamin B-Matrix Induced by RanGTP Required for Spindle Assembly SP - 1122771 KW - lamin KW - matrix AU - Tsai, Ming-Ying AU - Wang, Shusheng AU - Heidinger, Jill AU - Shumaker, Dale AU - Adam, Stephen AU - Goldman, Robert AU - Zheng, Yixian PY - 2006/03/16/ UR - http://dx.doi.org/10.1126/science.1122771 ER -