Genome-Wide Profiling of DNA Methylation Reveals Transposon Targets of CHROMOMETHYLASE3

@article{citeulike:2327830, abstract = {DNA methylation has been implicated in a variety of epigenetic processes, and abnormal methylation patterns have been seen in tumors [1, 2 and 3]. Analysis of methylation patterns has traditionally been conducted either by using Southern analysis after cleavage with methyl-sensitive restriction endonucleases or by bisulfite sequencing [4]. However, neither method is practical for analyzing more than a few genes. Here, we describe a simple technique for genome-wide mapping of DNA methylation patterns. Fragmentation by a methyl-sensitive restriction endonuclease is followed by size fractionation and hybridization to microarrays. We demonstrate the utility of this method by characterizing methylation patterns in Arabidopsis methylation mutants. This analysis reveals that CHROMOMETHYLASE3 (CMT3) [5], which was previously shown to maintain CpXpG methylation [6 and 7], preferentially methylates transposons, even when they are present as single copies within the genome. Methylation profiling has potential applications in disease research and diagnostic screening.}, author = {Tompa, Rachel and Mccallum, Claire M. and Delrow, Jeffrey and Henikoff, Jorja G. and van Steensel, Bas and Henikoff, Steven }, citeulike-article-id = {2327830}, doi = {10.1016/S0960-9822(01)00622-4}, journal = {Current Biology}, keywords = {methylation, transposon}, month = {January}, number = {1}, pages = {65--68}, posted-at = {2008-02-04 01:15:39}, priority = {2}, title = {Genome-Wide Profiling of DNA Methylation Reveals Transposon Targets of CHROMOMETHYLASE3}, url = {http://dx.doi.org/10.1016/S0960-9822(01)00622-4}, volume = {12}, year = {2002} }

TY - JOUR ID - citeulike:2327830 L3 - citeulike-article-id:2327830 TI - Genome-Wide Profiling of DNA Methylation Reveals Transposon Targets of CHROMOMETHYLASE3 JF - Current Biology VL - 12 IS - 1 SP - 65 EP - 68 N2 - DNA methylation has been implicated in a variety of epigenetic processes, and abnormal methylation patterns have been seen in tumors [1, 2 and 3]. Analysis of methylation patterns has traditionally been conducted either by using Southern analysis after cleavage with methyl-sensitive restriction endonucleases or by bisulfite sequencing [4]. However, neither method is practical for analyzing more than a few genes. Here, we describe a simple technique for genome-wide mapping of DNA methylation patterns. Fragmentation by a methyl-sensitive restriction endonuclease is followed by size fractionation and hybridization to microarrays. We demonstrate the utility of this method by characterizing methylation patterns in Arabidopsis methylation mutants. This analysis reveals that CHROMOMETHYLASE3 (CMT3) [5], which was previously shown to maintain CpXpG methylation [6 and 7], preferentially methylates transposons, even when they are present as single copies within the genome. Methylation profiling has potential applications in disease research and diagnostic screening. KW - methylation KW - transposon AU - Tompa, Rachel AU - Mccallum, Claire AU - Delrow, Jeffrey AU - Henikoff, Jorja AU - van Steensel, Bas AU - Henikoff, Steven PY - 2002/01/08/ UR - http://dx.doi.org/10.1016/S0960-9822(01)00622-4 ER -