The Alternative Splicing Gallery (ASG): bridging the gap between genome and transcriptome.

@article{citeulike:4990, abstract = {Alternative splicing essentially increases the diversity of the transcriptome and has important implications for physiology, development and the genesis of diseases. Conventionally, alternative splicing is investigated in a case-by-case fashion, but this becomes cumbersome and error prone if genes show a huge abundance of different splice variants. We use a different approach and integrate all transcripts derived from a gene into a single splicing graph. Each transcript corresponds to a path in the graph, and alternative splicing is displayed by bifurcations. This representation preserves the relationships between different splicing variants and allows us to investigate systematically all possible putative transcripts. We built a database of splicing graphs for human genes, using transcript information from various major sources (Ensembl, RefSeq, STACK, TIGR and UniGene). A Web interface allows users to display the splicing graphs, to interactively assemble transcripts and to access their sequences as well as neighboring genomic regions. We also provide for each gene an exhaustive pre-computed catalog of putative transcripts--in total more than 1.2 million sequences. We found that approximately 65\% of the investigated genes show evidence for alternative splicing, and in 5\% of the cases, a single gene might produce over 100 transcripts.}, address = {Department of Computer Science, College of Engineering, North Carolina State University, Raleigh, NC 27695-7566, USA.}, author = {Leipzig, J. and Pevzner, P. and Heber, S. }, citeulike-article-id = {4990}, doi = {10.1093/nar/gkh731}, issn = {1362-4962}, journal = {Nucleic Acids Res}, keywords = {alternative-splicing, genome-annotation}, number = {13}, pages = {3977--3983}, posted-at = {2007-08-08 13:54:51}, priority = {2}, title = {The Alternative Splicing Gallery (ASG): bridging the gap between genome and transcriptome.}, url = {http://dx.doi.org/10.1093/nar/gkh731}, volume = {32}, year = {2004} }

TY - JOUR ID - citeulike:4990 L3 - citeulike-article-id:4990 TI - The Alternative Splicing Gallery (ASG): bridging the gap between genome and transcriptome. JF - Nucleic Acids Res VL - 32 IS - 13 SP - 3977 EP - 3983 AD - Department of Computer Science, College of Engineering, North Carolina State University, Raleigh, NC 27695-7566, USA. SN - 1362-4962 N2 - Alternative splicing essentially increases the diversity of the transcriptome and has important implications for physiology, development and the genesis of diseases. Conventionally, alternative splicing is investigated in a case-by-case fashion, but this becomes cumbersome and error prone if genes show a huge abundance of different splice variants. We use a different approach and integrate all transcripts derived from a gene into a single splicing graph. Each transcript corresponds to a path in the graph, and alternative splicing is displayed by bifurcations. This representation preserves the relationships between different splicing variants and allows us to investigate systematically all possible putative transcripts. We built a database of splicing graphs for human genes, using transcript information from various major sources (Ensembl, RefSeq, STACK, TIGR and UniGene). A Web interface allows users to display the splicing graphs, to interactively assemble transcripts and to access their sequences as well as neighboring genomic regions. We also provide for each gene an exhaustive pre-computed catalog of putative transcripts--in total more than 1.2 million sequences. We found that approximately 65% of the investigated genes show evidence for alternative splicing, and in 5% of the cases, a single gene might produce over 100 transcripts. KW - alternative-splicing KW - genome-annotation AU - Leipzig, J AU - Pevzner, P AU - Heber, S PY - 2004/// UR - http://dx.doi.org/10.1093/nar/gkh731 ER -