In vitro selection and characterization of Bacillus anthracis mutants with high-level resistance to ciprofloxacin.

@article{citeulike:2089828, abstract = {Mutants of attenuated Bacillus anthracis with high-level ciprofloxacin resistance were isolated using a three-step in vitro selection. Ciprofloxacin MICs were 0.5 micro g/ml for first-step mutants, which had one of two gyrA quinolone resistance-determining region (QRDR) mutations. Ciprofloxacin MICs were 8 and 16 microg/ml for second-step mutants, which had one of three parC QRDR mutations. Ciprofloxacin MICs for third-step mutants were 32 and 64 microg/ml. Mutants for which MICs were 64 microg/ml had one of two additional mutations within the gyrA QRDR or one of two mutations within the gyrB QRDR. Mutants for which MICs were 32 microg/ml had no additional target modifications but showed evidence of enhanced ciprofloxacin efflux.}, address = {Department of Biological Sciences, Northern Arizona University, Flagstaff, Arizona 86011-5640, USA.}, author = {Price, L. B. and Vogler, A. and Pearson, T. and Busch, J. D. and Schupp, J. M. and Keim, P. }, citeulike-article-id = {2089828}, doi = {http://dx.doi.org/10.1128/AAC.47.7.2362-2365.2003}, issn = {0066-4804}, journal = {Antimicrob Agents Chemother}, keywords = {antibiotic-resistance, bacillus, microorganism-identification}, month = {July}, number = {7}, pages = {2362--2365}, posted-at = {2007-12-11 14:42:04}, priority = {2}, title = {In vitro selection and characterization of Bacillus anthracis mutants with high-level resistance to ciprofloxacin.}, url = {http://dx.doi.org/10.1128/AAC.47.7.2362-2365.2003}, volume = {47}, year = {2003} }

TY - JOUR ID - citeulike:2089828 L3 - citeulike-article-id:2089828 TI - In vitro selection and characterization of Bacillus anthracis mutants with high-level resistance to ciprofloxacin. JF - Antimicrob Agents Chemother VL - 47 IS - 7 SP - 2362 EP - 2365 AD - Department of Biological Sciences, Northern Arizona University, Flagstaff, Arizona 86011-5640, USA. SN - 0066-4804 N2 - Mutants of attenuated Bacillus anthracis with high-level ciprofloxacin resistance were isolated using a three-step in vitro selection. Ciprofloxacin MICs were 0.5 micro g/ml for first-step mutants, which had one of two gyrA quinolone resistance-determining region (QRDR) mutations. Ciprofloxacin MICs were 8 and 16 microg/ml for second-step mutants, which had one of three parC QRDR mutations. Ciprofloxacin MICs for third-step mutants were 32 and 64 microg/ml. Mutants for which MICs were 64 microg/ml had one of two additional mutations within the gyrA QRDR or one of two mutations within the gyrB QRDR. Mutants for which MICs were 32 microg/ml had no additional target modifications but showed evidence of enhanced ciprofloxacin efflux. KW - antibiotic-resistance KW - bacillus KW - microorganism-identification AU - Price, LB AU - Vogler, A AU - Pearson, T AU - Busch, JD AU - Schupp, JM AU - Keim, P PY - 2003/07// UR - http://dx.doi.org/10.1128/AAC.47.7.2362-2365.2003 ER -