Engineering stability in gene networks by autoregulation.

@article{citeulike:314, abstract = {The genetic and biochemical networks which underlie such things as homeostasis in metabolism and the developmental programs of living cells, must withstand considerable variations and random perturbations of biochemical parameters. These occur as transient changes in, for example, transcription, translation, and RNA and protein degradation. The intensity and duration of these perturbations differ between cells in a population. The unique state of cells, and thus the diversity in a population, is owing to the different environmental stimuli the individual cells experience and the inherent stochastic nature of biochemical processes (for example, refs 5 and 6). It has been proposed, but not demonstrated, that autoregulatory, negative feedback loops in gene circuits provide stability, thereby limiting the range over which the concentrations of network components fluctuate. Here we have designed and constructed simple gene circuits consisting of a regulator and transcriptional repressor modules in Escherichia coli and we show the gain of stability produced by negative feedback.}, address = {EMBL, Structures \& Biocomputing, Heidelberg, Germany. becskei@embl-heidelberg.de}, author = {Becskei, A. and Serrano, L. }, citeulike-article-id = {314}, doi = {10.1038/35014651}, issn = {0028-0836}, journal = {Nature}, keywords = {ecoli, fluctuations, gene-expression, noise, stochasticity}, month = {June}, number = {6786}, pages = {590--593}, posted-at = {2007-01-10 12:40:21}, priority = {0}, title = {Engineering stability in gene networks by autoregulation.}, url = {http://dx.doi.org/10.1038/35014651}, volume = {405}, year = {2000} }

TY - JOUR ID - citeulike:314 L3 - citeulike-article-id:314 TI - Engineering stability in gene networks by autoregulation. JF - Nature VL - 405 IS - 6786 SP - 590 EP - 593 AD - EMBL, Structures & Biocomputing, Heidelberg, Germany. becskei@embl-heidelberg.de SN - 0028-0836 N2 - The genetic and biochemical networks which underlie such things as homeostasis in metabolism and the developmental programs of living cells, must withstand considerable variations and random perturbations of biochemical parameters. These occur as transient changes in, for example, transcription, translation, and RNA and protein degradation. The intensity and duration of these perturbations differ between cells in a population. The unique state of cells, and thus the diversity in a population, is owing to the different environmental stimuli the individual cells experience and the inherent stochastic nature of biochemical processes (for example, refs 5 and 6). It has been proposed, but not demonstrated, that autoregulatory, negative feedback loops in gene circuits provide stability, thereby limiting the range over which the concentrations of network components fluctuate. Here we have designed and constructed simple gene circuits consisting of a regulator and transcriptional repressor modules in Escherichia coli and we show the gain of stability produced by negative feedback. KW - ecoli KW - fluctuations KW - gene-expression KW - noise KW - stochasticity AU - Becskei, A AU - Serrano, L PY - 2000/06/01/ UR - http://dx.doi.org/10.1038/35014651 ER -