Transient Reversal of RNA Polymerase II Active Site Closing Controls Fidelity of Transcription Elongation

@article{citeulike:2866443, abstract = {Summary To study fidelity of RNA polymerase II (Pol II), we analyzed properties of the 6-azauracil-sensitive and TFIIS-dependent E1103G mutant of rbp1 (rpo21), the gene encoding the catalytic subunit of Pol II in Saccharomyces cerevisiae. Using an in vivo retrotransposition-based transcription fidelity assay, we observed that rpb1-E1103G causes a 3-fold increase in transcription errors. This mutant showed a 10-fold decrease in fidelity of transcription elongation in vitro. The mutation does not appear to significantly affect translocation state equilibrium of Pol II in a stalled elongation complex. Primarily, it promotes NTP sequestration in the polymerase active center. Furthermore, pre-steady-state analyses revealed that the E1103G mutation shifted the equilibrium between the closed and the open active center conformations toward the closed form. Thus, open conformation of the active center emerges as an intermediate essential for preincorporation fidelity control. Similar mechanisms may control fidelity of DNA-dependent DNA polymerases and RNA-dependent RNA polymerases.}, author = {Kireeva, Maria L. and Nedialkov, Yuri A. and Cremona, Gina H. and Purtov, Yuri A. and Lubkowska, Lucyna and Malagon, Francisco and Burton, Zachary F. and Strathern, Jeffrey N. and Kashlev, Mikhail }, citeulike-article-id = {2866443}, doi = {http://dx.doi.org/10.1016/j.molcel.2008.04.017}, journal = {Molecular Cell}, keywords = {fidelity, transcription}, month = {June}, number = {5}, pages = {557--566}, posted-at = {2008-06-05 17:49:40}, priority = {2}, title = {Transient Reversal of RNA Polymerase II Active Site Closing Controls Fidelity of Transcription Elongation}, url = {http://dx.doi.org/10.1016/j.molcel.2008.04.017}, volume = {30}, year = {2008} }

TY - JOUR ID - citeulike:2866443 L3 - citeulike-article-id:2866443 N2 - Summary To study fidelity of RNA polymerase II (Pol II), we analyzed properties of the 6-azauracil-sensitive and TFIIS-dependent E1103G mutant of rbp1 (rpo21), the gene encoding the catalytic subunit of Pol II in Saccharomyces cerevisiae. Using an in vivo retrotransposition-based transcription fidelity assay, we observed that rpb1-E1103G causes a 3-fold increase in transcription errors. This mutant showed a 10-fold decrease in fidelity of transcription elongation in vitro. The mutation does not appear to significantly affect translocation state equilibrium of Pol II in a stalled elongation complex. Primarily, it promotes NTP sequestration in the polymerase active center. Furthermore, pre-steady-state analyses revealed that the E1103G mutation shifted the equilibrium between the closed and the open active center conformations toward the closed form. Thus, open conformation of the active center emerges as an intermediate essential for preincorporation fidelity control. Similar mechanisms may control fidelity of DNA-dependent DNA polymerases and RNA-dependent RNA polymerases. IS - 5 JF - Molecular Cell EP - 566 TI - Transient Reversal of RNA Polymerase II Active Site Closing Controls Fidelity of Transcription Elongation VL - 30 SP - 557 KW - fidelity KW - transcription AU - Kireeva, Maria AU - Nedialkov, Yuri AU - Cremona, Gina AU - Purtov, Yuri AU - Lubkowska, Lucyna AU - Malagon, Francisco AU - Burton, Zachary AU - Strathern, Jeffrey AU - Kashlev, Mikhail PY - 2008/06/05/ UR - http://dx.doi.org/10.1016/j.molcel.2008.04.017 ER -