Concurrent Fast and Slow Cycling of a Transcriptional Activator at an Endogenous Promoter

by: Tatiana S Karpova, Min J Kim, Corentin Spriet, Kip Nalley, Timothy J Stasevich, Zoulika Kherrouche, Laurent Heliot, James G Mcnally

Science, Vol. 319, No. 5862. (25 January 2008), pp. 466-469.

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摘要

For gene regulation, some transcriptional activators bind periodically to promoters with either a fast ([~]1 minute) or a slow ([~]15 to 90 minutes) cycle. It is uncertain whether the fast cycle occurs on natural promoters, and the function of either cycle in transcription remains unclear. We report that fast and slow cycling can occur simultaneously on an endogenous yeast promoter and that slow cycling in this system reflects an oscillation in the fraction of accessible promoters rather than the recruitment and release of stably bound transcriptional activators. This observation, combined with single-cell measurements of messenger RNA (mRNA) production, argues that fast cycling initiates transcription and that slow cycling regulates the quantity of mRNA produced. These findings counter the prevailing view that slow cycling initiates transcription. 10.1126/science.1150559

@article{citeulike:2288293, abstract = {For gene regulation, some transcriptional activators bind periodically to promoters with either a fast ([\~{}]1 minute) or a slow ([\~{}]15 to 90 minutes) cycle. It is uncertain whether the fast cycle occurs on natural promoters, and the function of either cycle in transcription remains unclear. We report that fast and slow cycling can occur simultaneously on an endogenous yeast promoter and that slow cycling in this system reflects an oscillation in the fraction of accessible promoters rather than the recruitment and release of stably bound transcriptional activators. This observation, combined with single-cell measurements of messenger RNA (mRNA) production, argues that fast cycling initiates transcription and that slow cycling regulates the quantity of mRNA produced. These findings counter the prevailing view that slow cycling initiates transcription. 10.1126/science.1150559}, author = {Karpova, Tatiana S. and Kim, Min J. and Spriet, Corentin and Nalley, Kip and Stasevich, Timothy J. and Kherrouche, Zoulika and Heliot, Laurent and Mcnally, James G. }, citeulike-article-id = {2288293}, doi = {http://dx.doi.org/10.1126/science.1150559}, journal = {Science}, keywords = {binding, dna, promoter, transcription, transcription-factor}, month = {January}, number = {5862}, pages = {466--469}, posted-at = {2008-01-30 10:55:28}, priority = {5}, title = {Concurrent Fast and Slow Cycling of a Transcriptional Activator at an Endogenous Promoter}, url = {http://dx.doi.org/10.1126/science.1150559}, volume = {319}, year = {2008} }

RIS record

TY - JOUR ID - citeulike:2288293 L3 - citeulike-article-id:2288293 N2 - For gene regulation, some transcriptional activators bind periodically to promoters with either a fast ([~]1 minute) or a slow ([~]15 to 90 minutes) cycle. It is uncertain whether the fast cycle occurs on natural promoters, and the function of either cycle in transcription remains unclear. We report that fast and slow cycling can occur simultaneously on an endogenous yeast promoter and that slow cycling in this system reflects an oscillation in the fraction of accessible promoters rather than the recruitment and release of stably bound transcriptional activators. This observation, combined with single-cell measurements of messenger RNA (mRNA) production, argues that fast cycling initiates transcription and that slow cycling regulates the quantity of mRNA produced. These findings counter the prevailing view that slow cycling initiates transcription. 10.1126/science.1150559 IS - 5862 JF - Science EP - 469 TI - Concurrent Fast and Slow Cycling of a Transcriptional Activator at an Endogenous Promoter VL - 319 SP - 466 KW - binding KW - dna KW - promoter KW - transcription KW - transcription-factor AU - Karpova, Tatiana AU - Kim, Min AU - Spriet, Corentin AU - Nalley, Kip AU - Stasevich, Timothy AU - Kherrouche, Zoulika AU - Heliot, Laurent AU - Mcnally, James PY - 2008/01/25/ UR - http://dx.doi.org/10.1126/science.1150559 ER -

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