Single-molecule tracking of mRNA exiting from RNA polymerase II.

@article{citeulike:2196366, abstract = {Single-pair fluorescence resonance energy transfer was used to track RNA exiting from RNA polymerase II (Pol II) in elongation complexes. Measuring the distance between the RNA 5' end and three known locations within the elongation complex allows us determine its position by means of triangulation. RNA leaves the polymerase active center cleft via the previously proposed exit tunnel and then disengages from the enzyme surface. When the RNA reaches lengths of 26 and 29 nt, its 5' end associates with Pol II at the base of the dock domain. Because the initiation factor TFIIB binds to the dock domain and exit tunnel, exiting RNA may prevent TFIIB reassociation during elongation. RNA further extends toward the linker connecting to the polymerase C-terminal repeat domain (CTD), which binds the 5'-capping enzyme and other RNA processing factors.}, address = {Munich Center for Integrated Protein Science (CiPS) and Center for Nanoscience (CeNS), Ludwig-Maximilians-Universit\"{a}t M\"{u}nchen, 81377 Munich, Germany;}, author = {Andrecka, Joanna and Lewis, Robert and Br\"{u}ckner, Florian and Lehmann, Elisabeth and Cramer, Patrick and Michaelis, Jens }, citeulike-article-id = {2196366}, doi = {http://dx.doi.org/10.1073/pnas.0703815105}, issn = {1091-6490}, journal = {Proc Natl Acad Sci U S A}, keywords = {mrnap, polymerase, rnap, transcription}, month = {December}, posted-at = {2008-01-09 11:21:50}, priority = {4}, title = {Single-molecule tracking of mRNA exiting from RNA polymerase II.}, url = {http://dx.doi.org/10.1073/pnas.0703815105}, year = {2007} }

TY - JOUR ID - citeulike:2196366 L3 - citeulike-article-id:2196366 N2 - Single-pair fluorescence resonance energy transfer was used to track RNA exiting from RNA polymerase II (Pol II) in elongation complexes. Measuring the distance between the RNA 5' end and three known locations within the elongation complex allows us determine its position by means of triangulation. RNA leaves the polymerase active center cleft via the previously proposed exit tunnel and then disengages from the enzyme surface. When the RNA reaches lengths of 26 and 29 nt, its 5' end associates with Pol II at the base of the dock domain. Because the initiation factor TFIIB binds to the dock domain and exit tunnel, exiting RNA may prevent TFIIB reassociation during elongation. RNA further extends toward the linker connecting to the polymerase C-terminal repeat domain (CTD), which binds the 5'-capping enzyme and other RNA processing factors. JF - Proc Natl Acad Sci U S A SN - 1091-6490 AD - Munich Center for Integrated Protein Science (CiPS) and Center for Nanoscience (CeNS), Ludwig-Maximilians-Universität München, 81377 Munich, Germany; TI - Single-molecule tracking of mRNA exiting from RNA polymerase II. KW - mrnap KW - polymerase KW - rnap KW - transcription AU - Andrecka, Joanna AU - Lewis, Robert AU - Brückner, Florian AU - Lehmann, Elisabeth AU - Cramer, Patrick AU - Michaelis, Jens PY - 2007/12/28/ UR - http://dx.doi.org/10.1073/pnas.0703815105 ER -