Gene regulation at the single-cell level.

@article{citeulike:141524, abstract = {The quantitative relation between transcription factor concentrations and the rate of protein production from downstream genes is central to the function of genetic networks. Here we show that this relation, which we call the gene regulation function (GRF), fluctuates dynamically in individual living cells, thereby limiting the accuracy with which transcriptional genetic circuits can transfer signals. Using fluorescent reporter genes and fusion proteins, we characterized the bacteriophage lambda promoter P(R) in Escherichia coli. A novel technique based on binomial errors in protein partitioning enabled calibration of in vivo biochemical parameters in molecular units. We found that protein production rates fluctuate over a time scale of about one cell cycle, while intrinsic noise decays rapidly. Thus, biochemical parameters, noise, and slowly varying cellular states together determine the effective single-cell GRF. These results can form a basis for quantitative modeling of natural gene circuits and for design of synthetic ones.}, address = {Departments of Molecular Cell Biology and Physics of Complex Systems, Weizmann Institute of Science, Rehovot, 76100, Israel.}, author = {Rosenfeld, N. and Young, J. W. and Alon, U. and Swain, P. S. and Elowitz, M. B. }, citeulike-article-id = {141524}, doi = {http://dx.doi.org/10.1126/science.1106914}, issn = {1095-9203}, journal = {Science}, keywords = {experiment, fluctuations, gene-expression, noise, single-cell, stochasticity}, month = {March}, number = {5717}, pages = {1962--1965}, posted-at = {2007-01-10 13:48:44}, priority = {0}, title = {Gene regulation at the single-cell level.}, url = {http://dx.doi.org/10.1126/science.1106914}, volume = {307}, year = {2005} }

TY - JOUR ID - citeulike:141524 L3 - citeulike-article-id:141524 N2 - The quantitative relation between transcription factor concentrations and the rate of protein production from downstream genes is central to the function of genetic networks. Here we show that this relation, which we call the gene regulation function (GRF), fluctuates dynamically in individual living cells, thereby limiting the accuracy with which transcriptional genetic circuits can transfer signals. Using fluorescent reporter genes and fusion proteins, we characterized the bacteriophage lambda promoter P(R) in Escherichia coli. A novel technique based on binomial errors in protein partitioning enabled calibration of in vivo biochemical parameters in molecular units. We found that protein production rates fluctuate over a time scale of about one cell cycle, while intrinsic noise decays rapidly. Thus, biochemical parameters, noise, and slowly varying cellular states together determine the effective single-cell GRF. These results can form a basis for quantitative modeling of natural gene circuits and for design of synthetic ones. IS - 5717 JF - Science SN - 1095-9203 AD - Departments of Molecular Cell Biology and Physics of Complex Systems, Weizmann Institute of Science, Rehovot, 76100, Israel. EP - 1965 TI - Gene regulation at the single-cell level. VL - 307 SP - 1962 KW - experiment KW - fluctuations KW - gene-expression KW - noise KW - single-cell KW - stochasticity AU - Rosenfeld, N AU - Young, JW AU - Alon, U AU - Swain, PS AU - Elowitz, MB PY - 2005/03/25/ UR - http://dx.doi.org/10.1126/science.1106914 ER -