A Network of Multiple Regulatory Layers Shapes Gene Expression in Fission Yeast

@article{citeulike:1381462, abstract = {Summary Gene expression is controlled at multiple layers, and cells may integrate different regulatory steps for coherent production of proper protein levels. We applied various microarray-based approaches to determine key gene-expression intermediates in exponentially growing fission yeast, providing genome-wide data for translational profiles, mRNA steady-state levels, polyadenylation profiles, start-codon sequence context, mRNA half-lives, and RNA polymerase II occupancy. We uncovered widespread and unexpected relationships between distinct aspects of gene expression. Translation and polyadenylation are aligned on a global scale with both the lengths and levels of mRNAs: efficiently translated mRNAs have longer poly(A) tails and are shorter, more stable, and more efficiently transcribed on average. Transcription and translation may be independently but congruently optimized to streamline protein production. These rich data sets, all acquired under a standardized condition, reveal a substantial coordination between regulatory layers and provide a basis for a systems-level understanding of multilayered gene-expression programs.}, author = {Lackner, Daniel H. and Beilharz, Traude H. and Marguerat, Samuel and Mata, Juan and Watt, Stephen and Schubert, Falk and Preiss, Thomas and Bahler, Jurg }, citeulike-article-id = {1381462}, journal = {Molecular Cell}, keywords = {experiment, gene-expression, gene-regulation, yeast}, month = {April}, number = {1}, pages = {145--155}, posted-at = {2007-06-12 14:28:11}, priority = {0}, title = {A Network of Multiple Regulatory Layers Shapes Gene Expression in Fission Yeast}, url = {http://www.sciencedirect.com/science/article/B6WSR-4NGCY1R-D/2/9cac71f732a8b41bb8c073c99fab57c0}, volume = {26}, year = {2007} }

TY - JOUR ID - citeulike:1381462 L3 - citeulike-article-id:1381462 N2 - Summary Gene expression is controlled at multiple layers, and cells may integrate different regulatory steps for coherent production of proper protein levels. We applied various microarray-based approaches to determine key gene-expression intermediates in exponentially growing fission yeast, providing genome-wide data for translational profiles, mRNA steady-state levels, polyadenylation profiles, start-codon sequence context, mRNA half-lives, and RNA polymerase II occupancy. We uncovered widespread and unexpected relationships between distinct aspects of gene expression. Translation and polyadenylation are aligned on a global scale with both the lengths and levels of mRNAs: efficiently translated mRNAs have longer poly(A) tails and are shorter, more stable, and more efficiently transcribed on average. Transcription and translation may be independently but congruently optimized to streamline protein production. These rich data sets, all acquired under a standardized condition, reveal a substantial coordination between regulatory layers and provide a basis for a systems-level understanding of multilayered gene-expression programs. IS - 1 JF - Molecular Cell EP - 155 TI - A Network of Multiple Regulatory Layers Shapes Gene Expression in Fission Yeast VL - 26 SP - 145 KW - experiment KW - gene-expression KW - gene-regulation KW - yeast AU - Lackner, Daniel AU - Beilharz, Traude AU - Marguerat, Samuel AU - Mata, Juan AU - Watt, Stephen AU - Schubert, Falk AU - Preiss, Thomas AU - Bahler, Jurg PY - 2007/04/13/ UR - http://www.sciencedirect.com/science/article/B6WSR-4NGCY1R-D/2/9cac71f732a8b41bb8c073c99fab57c0 ER -