Visualizing Spatiotemporal Dynamics of Multicellular Cell-Cycle Progression

@article{citeulike:2354122, abstract = {Summary The cell-cycle transition from G1 to S phase has been difficult to visualize. We have harnessed antiphase oscillating proteins that mark cell-cycle transitions in order to develop genetically encoded fluorescent probes for this purpose. These probes effectively label individual G1 phase nuclei red and those in S/G2/M phases green. We were able to generate cultured cells and transgenic mice constitutively expressing the cell-cycle probes, in which every cell nucleus exhibits either red or green fluorescence. We performed time-lapse imaging to explore the spatiotemporal patterns of cell-cycle dynamics during the epithelial-mesenchymal transition of cultured cells, the migration and differentiation of neural progenitors in brain slices, and the development of tumors across blood vessels in live mice. These mice and cell lines will serve as model systems permitting unprecedented spatial and temporal resolution to help us better understand how the cell cycle is coordinated with various biological events.}, author = {Sakaue-Sawano, Asako and Kurokawa, Hiroshi and Morimura, Toshifumi and Hanyu, Aki and Hama, Hiroshi and Osawa, Hatsuki and Kashiwagi, Saori and Fukami, Kiyoko and Miyata, Takaki and Miyoshi, Hiroyuki and Imamura, Takeshi and Ogawa, Masaharu and Masai, Hisao and Miyawaki, Atsushi }, citeulike-article-id = {2354122}, doi = {10.1016/j.cell.2007.12.033}, journal = {Cell}, keywords = {cell-cycle, imaging, regulation}, month = {February}, number = {3}, pages = {487--498}, posted-at = {2008-04-06 15:38:33}, priority = {0}, title = {Visualizing Spatiotemporal Dynamics of Multicellular Cell-Cycle Progression}, url = {http://dx.doi.org/10.1016/j.cell.2007.12.033}, volume = {132}, year = {2008} }

TY - JOUR ID - citeulike:2354122 L3 - citeulike-article-id:2354122 TI - Visualizing Spatiotemporal Dynamics of Multicellular Cell-Cycle Progression JF - Cell VL - 132 IS - 3 SP - 487 EP - 498 N2 - Summary The cell-cycle transition from G1 to S phase has been difficult to visualize. We have harnessed antiphase oscillating proteins that mark cell-cycle transitions in order to develop genetically encoded fluorescent probes for this purpose. These probes effectively label individual G1 phase nuclei red and those in S/G2/M phases green. We were able to generate cultured cells and transgenic mice constitutively expressing the cell-cycle probes, in which every cell nucleus exhibits either red or green fluorescence. We performed time-lapse imaging to explore the spatiotemporal patterns of cell-cycle dynamics during the epithelial-mesenchymal transition of cultured cells, the migration and differentiation of neural progenitors in brain slices, and the development of tumors across blood vessels in live mice. These mice and cell lines will serve as model systems permitting unprecedented spatial and temporal resolution to help us better understand how the cell cycle is coordinated with various biological events. KW - cell-cycle KW - imaging KW - regulation AU - Sakaue-Sawano, Asako AU - Kurokawa, Hiroshi AU - Morimura, Toshifumi AU - Hanyu, Aki AU - Hama, Hiroshi AU - Osawa, Hatsuki AU - Kashiwagi, Saori AU - Fukami, Kiyoko AU - Miyata, Takaki AU - Miyoshi, Hiroyuki AU - Imamura, Takeshi AU - Ogawa, Masaharu AU - Masai, Hisao AU - Miyawaki, Atsushi PY - 2008/02/08/ UR - http://dx.doi.org/10.1016/j.cell.2007.12.033 ER -