Genomic DNA functions as a universal external standard in quantitative real-time PCR.

@article{citeulike:1449569, abstract = {Real-time quantitative PCR (qPCR) is a powerful tool for quantifying specific DNA target sequences. Although determination of relative quantity is widely accepted as a reliable means of measuring differences between samples, there are advantages to being able to determine the absolute copy numbers of a given target. One approach to absolute quantification relies on construction of an accurate standard curve using appropriate external standards of known concentration. We have validated the use of tissue genomic DNA as a universal external standard to facilitate quantification of any target sequence contained in the genome of a given species, addressing several key technical issues regarding its use. This approach was applied to validate mRNA expression of gene candidates identified from microarray data and to determine gene copies in transgenic mice. A simple method that can assist achieving absolute quantification of gene expression would broadly enhance the uses of real-time qPCR and in particular, augment the evaluation of global gene expression studies.}, address = {Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario, Canada M5S 1A8.}, author = {Yun, J. J. and Heisler, L. E. and Hwang, I. I. and Wilkins, O. and Lau, S. K. and Hyrcza, M. and Jayabalasingham, B. and Jin, J. and McLaurin, J. and Tsao, M. S. and Der, S. D. }, citeulike-article-id = {1449569}, issn = {1362-4962}, journal = {Nucleic Acids Res}, number = {12}, posted-at = {2007-07-11 16:55:26}, priority = {2}, title = {Genomic DNA functions as a universal external standard in quantitative real-time PCR.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/16840529}, volume = {34}, year = {2006} }

TY - JOUR ID - citeulike:1449569 L3 - citeulike-article-id:1449569 TI - Genomic DNA functions as a universal external standard in quantitative real-time PCR. JF - Nucleic Acids Res VL - 34 IS - 12 AD - Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario, Canada M5S 1A8. SN - 1362-4962 N2 - Real-time quantitative PCR (qPCR) is a powerful tool for quantifying specific DNA target sequences. Although determination of relative quantity is widely accepted as a reliable means of measuring differences between samples, there are advantages to being able to determine the absolute copy numbers of a given target. One approach to absolute quantification relies on construction of an accurate standard curve using appropriate external standards of known concentration. We have validated the use of tissue genomic DNA as a universal external standard to facilitate quantification of any target sequence contained in the genome of a given species, addressing several key technical issues regarding its use. This approach was applied to validate mRNA expression of gene candidates identified from microarray data and to determine gene copies in transgenic mice. A simple method that can assist achieving absolute quantification of gene expression would broadly enhance the uses of real-time qPCR and in particular, augment the evaluation of global gene expression studies. AU - Yun, JJ AU - Heisler, LE AU - Hwang, II AU - Wilkins, O AU - Lau, SK AU - Hyrcza, M AU - Jayabalasingham, B AU - Jin, J AU - McLaurin, J AU - Tsao, MS AU - Der, SD PY - 2006/// UR - http://view.ncbi.nlm.nih.gov/pubmed/16840529 ER -