Functional Genomic Analysis of the Wnt-Wingless Signaling Pathway.

@article{citeulike:179593, abstract = {The Wnt-Wingless (Wg) pathway is one of a core set of evolutionarily conserved signaling pathways that regulates many aspects of metazoan development. Aberrant Wnt signaling has been linked to human disease. In the present study we used a genomewide RNA interference (RNAi) screen in Drosophila cells to screen for regulators of the Wnt pathway. We identified 238 potential regulators, which include known pathway components, genes with functions not previously linked to this pathway, and genes with no previously assigned functions. Reciprocal-Best-Blast analyses reveal that 50\% of the genes identified in the screen have human orthologs of which \~{}18\% are associated with human disease. Functional assays of selected genes from the cell-based screen in Drosophila, mammalian cells, and zebrafish embryos demonstrated that these genes have evolutionarily conserved functions in Wnt signaling. High throughput RNAi screens in cultured cells, followed by functional analyses in model organisms, proves to be a rapid means of identifying regulators of signaling pathways implicated in development and disease.}, address = {Department of Genetics, Howard Hughes Medical Institute (HHMI), Harvard Medical School, NRB no. 339, 77 Avenue Louis Pasteur, Boston, MA 02115, USA.}, author = {Dasgupta, Ramanuj and Kaykas, Ajamete and Moon, Randall T T. and Perrimon, Norbert }, citeulike-article-id = {179593}, doi = {http://dx.doi.org/10.1126/science.1109374}, issn = {1095-9203}, journal = {Science}, keywords = {phenotype\_review}, month = {April}, posted-at = {2008-06-16 21:15:20}, priority = {2}, title = {Functional Genomic Analysis of the Wnt-Wingless Signaling Pathway.}, url = {http://dx.doi.org/10.1126/science.1109374}, year = {2005} }

TY - JOUR ID - citeulike:179593 L3 - citeulike-article-id:179593 TI - Functional Genomic Analysis of the Wnt-Wingless Signaling Pathway. JF - Science AD - Department of Genetics, Howard Hughes Medical Institute (HHMI), Harvard Medical School, NRB no. 339, 77 Avenue Louis Pasteur, Boston, MA 02115, USA. SN - 1095-9203 N2 - The Wnt-Wingless (Wg) pathway is one of a core set of evolutionarily conserved signaling pathways that regulates many aspects of metazoan development. Aberrant Wnt signaling has been linked to human disease. In the present study we used a genomewide RNA interference (RNAi) screen in Drosophila cells to screen for regulators of the Wnt pathway. We identified 238 potential regulators, which include known pathway components, genes with functions not previously linked to this pathway, and genes with no previously assigned functions. Reciprocal-Best-Blast analyses reveal that 50% of the genes identified in the screen have human orthologs of which ~18% are associated with human disease. Functional assays of selected genes from the cell-based screen in Drosophila, mammalian cells, and zebrafish embryos demonstrated that these genes have evolutionarily conserved functions in Wnt signaling. High throughput RNAi screens in cultured cells, followed by functional analyses in model organisms, proves to be a rapid means of identifying regulators of signaling pathways implicated in development and disease. KW - phenotype_review AU - Dasgupta, Ramanuj AU - Kaykas, Ajamete AU - Moon, Randall T AU - Perrimon, Norbert PY - 2005/04/07/ UR - http://dx.doi.org/10.1126/science.1109374 ER -