TWO-PHOTON EXCITATION FLUORESCENCE MICROSCOPY

by: Peter TC So, Chen Y Dong, Barry R Masters, Keith M Berland

Annual Review of Biomedical Engineering, Vol. 2, No. 1. (2000), pp. 399-429.

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摘要

Abstract Two-photon fluorescence microscopy is one of the most important recent inventions in biological imaging. This technology enables noninvasive study of biological specimens in three dimensions with submicrometer resolution. Two-photon excitation of fluorophores results from the simultaneous absorption of two photons. This excitation process has a number of unique advantages, such as reduced specimen photodamage and enhanced penetration depth. It also produces higher-contrast images and is a novel method to trigger localized photochemical reactions. Two-photon microscopy continues to find an increasing number of applications in biology and medicine.

@article{citeulike:2705308, abstract = {Abstract Two-photon fluorescence microscopy is one of the most important recent inventions in biological imaging. This technology enables noninvasive study of biological specimens in three dimensions with submicrometer resolution. Two-photon excitation of fluorophores results from the simultaneous absorption of two photons. This excitation process has a number of unique advantages, such as reduced specimen photodamage and enhanced penetration depth. It also produces higher-contrast images and is a novel method to trigger localized photochemical reactions. Two-photon microscopy continues to find an increasing number of applications in biology and medicine.}, author = {So, Peter T. C. and Dong, Chen Y. and Masters, Barry R. and Berland, Keith M. }, citeulike-article-id = {2705308}, doi = {10.1146/annurev.bioeng.2.1.399}, journal = {Annual Review of Biomedical Engineering}, keywords = {microscopy, multiphoton}, number = {1}, pages = {399--429}, posted-at = {2008-04-23 05:45:09}, priority = {2}, title = {TWO-PHOTON EXCITATION FLUORESCENCE MICROSCOPY}, url = {http://arjournals.annualreviews.org/doi/abs/10.1146/annurev.bioeng.2.1.399}, volume = {2}, year = {2000} }

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TY - JOUR ID - citeulike:2705308 L3 - citeulike-article-id:2705308 TI - TWO-PHOTON EXCITATION FLUORESCENCE MICROSCOPY JF - Annual Review of Biomedical Engineering VL - 2 IS - 1 SP - 399 EP - 429 N2 - Abstract Two-photon fluorescence microscopy is one of the most important recent inventions in biological imaging. This technology enables noninvasive study of biological specimens in three dimensions with submicrometer resolution. Two-photon excitation of fluorophores results from the simultaneous absorption of two photons. This excitation process has a number of unique advantages, such as reduced specimen photodamage and enhanced penetration depth. It also produces higher-contrast images and is a novel method to trigger localized photochemical reactions. Two-photon microscopy continues to find an increasing number of applications in biology and medicine. KW - microscopy KW - multiphoton AU - So, Peter AU - Dong, Chen AU - Masters, Barry AU - Berland, Keith PY - 2000/// UR - http://arjournals.annualreviews.org/doi/abs/10.1146/annurev.bioeng.2.1.399 ER -

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