Structural Insight into Epitopes in the Pregnancy-Associated Malaria Protein VAR2CSA

@article{citeulike:3288238, abstract = {Pregnancy-associated malaria is caused by Plasmodium falciparum malaria parasites binding specifically to chondroitin sulfate A in the placenta. This sequestration of parasites is a major cause of low birth weight in infants and anemia in the mothers. VAR2CSA, a polymorphic multi-domain protein of the PfEMP1 family, is the main parasite ligand for CSA binding, and identification of protective antibody epitopes is essential for VAR2CSA vaccine development. Attempts to determine the crystallographic structures of VAR2CSA or its domains have not been successful yet. In this study, we propose 3D models for each of the VAR2CSA DBL domains and we show that regions in the fold of VAR2CSA inter-domain 2 and a PfEMP1 CIDR domain seem to be homologous to the EBA-175 and Pkα-DBL fold. This suggests that ID2 could be a functional domain. We also identify regions of VAR2CSA present on the surface of native VAR2CSA by comparing reactivity of plasma containing anti-VAR2CSA antibodies in peptide array experiments before and after incubation with native VAR2CSA. By this method we identify conserved VAR2CSA regions targeted by antibodies that react with the native molecule expressed on infected erythrocytes. By mapping the data onto the DBL models we present evidence suggesting that the S1+S2 DBL sub-domains are generally surface-exposed in most domains, whereas the S3 sub-domains are less exposed in native VAR2CSA. These results comprise an important step towards understanding the structure of VAR2CSA on the surface of CSA-binding infected erythrocytes.}, author = {Andersen, Pernille and Nielsen, Morten A. and Resende, Mafalda and Rask, Thomas S. and Dahlb\"{a}ck, Madeleine and Theander, Thor and Lund, Ole and Salanti, Ali }, citeulike-article-id = {3288238}, doi = {http://dx.doi.org/10.1371/journal.ppat.0040042}, journal = {PLoS Pathog}, keywords = {mmult-ref}, month = {February}, number = {2}, pages = {e42+}, posted-at = {2008-09-18 16:57:21}, priority = {2}, publisher = {Public Library of Science}, title = {Structural Insight into Epitopes in the Pregnancy-Associated Malaria Protein VAR2CSA}, url = {http://dx.doi.org/10.1371/journal.ppat.0040042}, volume = {4}, year = {2008} }

TY - JOUR ID - citeulike:3288238 L3 - citeulike-article-id:3288238 N2 - Pregnancy-associated malaria is caused by Plasmodium falciparum malaria parasites binding specifically to chondroitin sulfate A in the placenta. This sequestration of parasites is a major cause of low birth weight in infants and anemia in the mothers. VAR2CSA, a polymorphic multi-domain protein of the PfEMP1 family, is the main parasite ligand for CSA binding, and identification of protective antibody epitopes is essential for VAR2CSA vaccine development. Attempts to determine the crystallographic structures of VAR2CSA or its domains have not been successful yet. In this study, we propose 3D models for each of the VAR2CSA DBL domains and we show that regions in the fold of VAR2CSA inter-domain 2 and a PfEMP1 CIDR domain seem to be homologous to the EBA-175 and Pkα-DBL fold. This suggests that ID2 could be a functional domain. We also identify regions of VAR2CSA present on the surface of native VAR2CSA by comparing reactivity of plasma containing anti-VAR2CSA antibodies in peptide array experiments before and after incubation with native VAR2CSA. By this method we identify conserved VAR2CSA regions targeted by antibodies that react with the native molecule expressed on infected erythrocytes. By mapping the data onto the DBL models we present evidence suggesting that the S1+S2 DBL sub-domains are generally surface-exposed in most domains, whereas the S3 sub-domains are less exposed in native VAR2CSA. These results comprise an important step towards understanding the structure of VAR2CSA on the surface of CSA-binding infected erythrocytes. IS - 2 JF - PLoS Pathog TI - Structural Insight into Epitopes in the Pregnancy-Associated Malaria Protein VAR2CSA PB - Public Library of Science VL - 4 SP - e42 KW - mmult-ref AU - Andersen, Pernille AU - Nielsen, Morten AU - Resende, Mafalda AU - Rask, Thomas AU - Dahlbäck, Madeleine AU - Theander, Thor AU - Lund, Ole AU - Salanti, Ali PY - 2008/02/15/ UR - http://dx.doi.org/10.1371/journal.ppat.0040042 ER -