Anaphase Inactivation of the Spindle Checkpoint

by: William J Palframan, Janet B Meehl, Sue L Jaspersen, Mark Winey, Andrew W Murray

Science, Vol. 313, No. 5787. (4 August 2006), pp. 680-684.

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摘要

The spindle checkpoint delays cell cycle progression until microtubules attach each pair of sister chromosomes to opposite poles of the mitotic spindle. Following sister chromatid separation, however, the checkpoint ignores chromosomes whose kinetochores are attached to only one spindle pole, a state that activates the checkpoint prior to metaphase. We demonstrate that, in budding yeast, mutual inhibition between the anaphase-promoting complex (APC) and Mps1, an essential component of the checkpoint, leads to sustained inactivation of the spindle checkpoint. Mps1 protein abundance decreases in anaphase, and Mps1 is a target of the APC. Furthermore, expression of Mps1 in anaphase, or repression of the APC in anaphase, reactivates the spindle checkpoint. This APC-Mps1 feedback circuit allows cells to irreversibly inactivate the checkpoint during anaphase. 10.1126/science.1127205

@article{citeulike:2732448, abstract = {The spindle checkpoint delays cell cycle progression until microtubules attach each pair of sister chromosomes to opposite poles of the mitotic spindle. Following sister chromatid separation, however, the checkpoint ignores chromosomes whose kinetochores are attached to only one spindle pole, a state that activates the checkpoint prior to metaphase. We demonstrate that, in budding yeast, mutual inhibition between the anaphase-promoting complex (APC) and Mps1, an essential component of the checkpoint, leads to sustained inactivation of the spindle checkpoint. Mps1 protein abundance decreases in anaphase, and Mps1 is a target of the APC. Furthermore, expression of Mps1 in anaphase, or repression of the APC in anaphase, reactivates the spindle checkpoint. This APC-Mps1 feedback circuit allows cells to irreversibly inactivate the checkpoint during anaphase. 10.1126/science.1127205}, author = {Palframan, William J. and Meehl, Janet B. and Jaspersen, Sue L. and Winey, Mark and Murray, Andrew W. }, citeulike-article-id = {2732448}, doi = {http://dx.doi.org/10.1126/science.1127205}, journal = {Science}, keywords = {mitosis}, month = {August}, number = {5787}, pages = {680--684}, posted-at = {2008-04-29 09:26:21}, priority = {2}, title = {Anaphase Inactivation of the Spindle Checkpoint}, url = {http://dx.doi.org/10.1126/science.1127205}, volume = {313}, year = {2006} }

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TY - JOUR ID - citeulike:2732448 L3 - citeulike-article-id:2732448 TI - Anaphase Inactivation of the Spindle Checkpoint JF - Science VL - 313 IS - 5787 SP - 680 EP - 684 N2 - The spindle checkpoint delays cell cycle progression until microtubules attach each pair of sister chromosomes to opposite poles of the mitotic spindle. Following sister chromatid separation, however, the checkpoint ignores chromosomes whose kinetochores are attached to only one spindle pole, a state that activates the checkpoint prior to metaphase. We demonstrate that, in budding yeast, mutual inhibition between the anaphase-promoting complex (APC) and Mps1, an essential component of the checkpoint, leads to sustained inactivation of the spindle checkpoint. Mps1 protein abundance decreases in anaphase, and Mps1 is a target of the APC. Furthermore, expression of Mps1 in anaphase, or repression of the APC in anaphase, reactivates the spindle checkpoint. This APC-Mps1 feedback circuit allows cells to irreversibly inactivate the checkpoint during anaphase. 10.1126/science.1127205 KW - mitosis AU - Palframan, William AU - Meehl, Janet AU - Jaspersen, Sue AU - Winey, Mark AU - Murray, Andrew PY - 2006/August/04/ UR - http://dx.doi.org/10.1126/science.1127205 ER -

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