Wave front engineering for microscopy of living cells

@article{citeulike:2663952, abstract = {A new method to perform simultaneously three dimensional optical sectioning and optical manipulation is presented. The system combines a multi trap optical tweezers with a video microscope to enable axial scanning of living cells while maintaining the trapping configuration at a fixed position. This is achieved compensating the axial movement of the objective by shaping the wave front of the trapping beam with properly diffractive optical elements displayed on a computer controlled spatial light modulator. Our method has been validated in three different experimental configurations. In the first, we decouple the position of a trapping plane from the axial movements of the objective and perform optical sectioning of a circle of beads kept on a fixed plane. In a second experiment, we extend the method to living cell microscopy by showing that mechanical constraints can be applied on the dorsal surface of a cell whilst performing its fluorescence optical sectioning. In the third experiment, we trapped beads in a three dimensional geometry and perform, always through the same objective, an axial scan of the volume delimited by the beads.}, author = {Emiliani, Valentina and Cojoc, Dan and Ferrari, Enrico and Garbin, Valeria and Durieux, Christiane and Moisan, Maite C. and Di Fabrizio, Enzo }, citeulike-article-id = {2663952}, journal = {Optics Express}, keywords = {tweezers}, month = {March}, number = {5}, pages = {1395--1405}, posted-at = {2008-04-13 16:44:17}, priority = {2}, publisher = {OSA}, title = {Wave front engineering for microscopy of living cells}, url = {http://www.opticsinfobase.org/abstract.cfm?id=82892}, volume = {13}, year = {2005} }

TY - JOUR ID - citeulike:2663952 L3 - citeulike-article-id:2663952 TI - Wave front engineering for microscopy of living cells JF - Optics Express VL - 13 IS - 5 SP - 1395 EP - 1405 PB - OSA N2 - A new method to perform simultaneously three dimensional optical sectioning and optical manipulation is presented. The system combines a multi trap optical tweezers with a video microscope to enable axial scanning of living cells while maintaining the trapping configuration at a fixed position. This is achieved compensating the axial movement of the objective by shaping the wave front of the trapping beam with properly diffractive optical elements displayed on a computer controlled spatial light modulator. Our method has been validated in three different experimental configurations. In the first, we decouple the position of a trapping plane from the axial movements of the objective and perform optical sectioning of a circle of beads kept on a fixed plane. In a second experiment, we extend the method to living cell microscopy by showing that mechanical constraints can be applied on the dorsal surface of a cell whilst performing its fluorescence optical sectioning. In the third experiment, we trapped beads in a three dimensional geometry and perform, always through the same objective, an axial scan of the volume delimited by the beads. KW - tweezers AU - Emiliani, Valentina AU - Cojoc, Dan AU - Ferrari, Enrico AU - Garbin, Valeria AU - Durieux, Christiane AU - Moisan, Maite AU - Di Fabrizio, Enzo PY - 2005/March/07/ UR - http://www.opticsinfobase.org/abstract.cfm?id=82892 ER -