Kinetochore microtubule dynamics and attachment stability are regulated by Hec1.

@article{citeulike:1428325, abstract = {Mitotic cells face the challenging tasks of linking kinetochores to growing and shortening microtubules and actively regulating these dynamic attachments to produce accurate chromosome segregation. We report here that Ndc80/Hec1 functions in regulating kinetochore microtubule plus-end dynamics and attachment stability. Microinjection of an antibody to the N terminus of Hec1 suppresses both microtubule detachment and microtubule plus-end polymerization and depolymerization at kinetochores of PtK1 cells. Centromeres become hyperstretched, kinetochore fibers shorten from spindle poles, kinetochore microtubule attachment errors increase, and chromosomes severely mis-segregate. The N terminus of Hec1 is phosphorylated by Aurora B kinase in vitro, and cells expressing N-terminal nonphosphorylatable mutants of Hec1 exhibit an increase in merotelic attachments, hyperstretching of centromeres, and errors in chromosome segregation. These findings reveal a key role for the Hec1 N terminus in controlling dynamic behavior of kinetochore microtubules.}, address = {Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA. jgdeluca@email.unc.edu}, author = {Deluca, J. G. and Gall, W. E. and Ciferri, C. and Cimini, D. and Musacchio, A. and Salmon, E. D. }, citeulike-article-id = {1428325}, doi = {10.1016/j.cell.2006.09.047}, issn = {0092-8674}, journal = {Cell}, keywords = {mitosis}, month = {December}, number = {5}, pages = {969--982}, posted-at = {2008-04-29 09:23:51}, priority = {2}, title = {Kinetochore microtubule dynamics and attachment stability are regulated by Hec1.}, url = {http://dx.doi.org/10.1016/j.cell.2006.09.047}, volume = {127}, year = {2006} }

TY - JOUR ID - citeulike:1428325 L3 - citeulike-article-id:1428325 TI - Kinetochore microtubule dynamics and attachment stability are regulated by Hec1. JF - Cell VL - 127 IS - 5 SP - 969 EP - 982 AD - Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA. jgdeluca@email.unc.edu SN - 0092-8674 N2 - Mitotic cells face the challenging tasks of linking kinetochores to growing and shortening microtubules and actively regulating these dynamic attachments to produce accurate chromosome segregation. We report here that Ndc80/Hec1 functions in regulating kinetochore microtubule plus-end dynamics and attachment stability. Microinjection of an antibody to the N terminus of Hec1 suppresses both microtubule detachment and microtubule plus-end polymerization and depolymerization at kinetochores of PtK1 cells. Centromeres become hyperstretched, kinetochore fibers shorten from spindle poles, kinetochore microtubule attachment errors increase, and chromosomes severely mis-segregate. The N terminus of Hec1 is phosphorylated by Aurora B kinase in vitro, and cells expressing N-terminal nonphosphorylatable mutants of Hec1 exhibit an increase in merotelic attachments, hyperstretching of centromeres, and errors in chromosome segregation. These findings reveal a key role for the Hec1 N terminus in controlling dynamic behavior of kinetochore microtubules. KW - mitosis AU - Deluca, JG AU - Gall, WE AU - Ciferri, C AU - Cimini, D AU - Musacchio, A AU - Salmon, ED PY - 2006/December/01/ UR - http://dx.doi.org/10.1016/j.cell.2006.09.047 ER -