Spontaneous platelet aggregation in normal subject assessed by a laser light scattering method: An attempt at standardization

@article{citeulike:2840062, abstract = {Platelet aggregometry by the laser light scattering (LS) method is sufficiently sensitive to detect small platelet aggregates that form spontaneously in vitro in the absence of agonists. Platelet aggregation without agonists is named spontaneous platelet aggregation (SPA). Since SPA has been suggested to be associated with various thrombotic diseases, it is essential to measure SPA and to establish a standard range of SPA values. In this study, we measured SPA in 167 healthy subjects by the LS method and attempted to clarify various factors influencing SPA, including the blood collection procedure. We also attempted to establish a tentative standard range of SPA values. SPA was quantitatively measured in terms of the maximum total LS intensity, which reflects small aggregates formed over 10 minutes (SMAX) and the area under the total LS intensity curve of small aggregates (SAUC). Since both the values of SMAX and SAUC were skewed and the log SMAX and log AUC values showed a normal distribution, the statistical analyses were performed using log SMAX and log SAUC. The log SMAX and log SAUC were significantly higher in the samples collected using a tourniquet and/or a 21 G needle, than in those collected without a tourniquet and/or with an 18 G needle. The log SAUC values were significantly lower in samples obtained with a syringe and/or 3.8\% sodium citrate than in those obtained in vacuum sampling tubes and/or 3.13\% or 3.14\% sodium citrate. The Ht and plasma glucose concentration influenced the log SMAX values. We propose that to standardize SPA measurements, the measurements should be completed within two hours of blood sample collection and collected using the regular concentration of citrate. The standard range of SMAX values measured in samples obtained using a tourniquet and a 21 G needle was 2.0–23.99 (*10³ mV*count). The standard range of SAUC values measured under same conditions was 0.58–9.12 (*10⁶ mV*count*min). The standard range of SMAX values measured in samples obtained using a tourniquet, 21 G needle and a vacuum tube was 1.7–29.51 (*10³ mV*count). The standard range of SAUC values measured under same conditions was 0.59–9.33 (*10⁶ mV*count*min).}, author = {Toshima, Hiroko and Sugihara, Hiroshi and Hamano, Hitoshi and Sato, Masashi and Yamamoto, Masahiro and Yamazaki, Satoshi and Yamada, Yukio and Taki, Masashi and Izumi, Shun-Ichiro and Hoshi, Keiko and Fusegawa, Yuichi and Satoh, Kaneo and Ozaki, Yukio and Kurihara, Satoshi }, citeulike-article-id = {2840062}, doi = {http://dx.doi.org/10.1080/09537100701883457}, journal = {Platelets}, keywords = {aggregation, laser, platelet, technology}, number = {4}, pages = {293--299}, posted-at = {2008-05-28 09:27:45}, priority = {2}, publisher = {Informa Healthcare}, title = {Spontaneous platelet aggregation in normal subject assessed by a laser light scattering method: An attempt at standardization}, url = {http://dx.doi.org/10.1080/09537100701883457}, volume = {19}, year = {2008} }

TY - JOUR ID - citeulike:2840062 L3 - citeulike-article-id:2840062 TI - Spontaneous platelet aggregation in normal subject assessed by a laser light scattering method: An attempt at standardization JF - Platelets VL - 19 IS - 4 SP - 293 EP - 299 PB - Informa Healthcare N2 - Platelet aggregometry by the laser light scattering (LS) method is sufficiently sensitive to detect small platelet aggregates that form spontaneously in vitro in the absence of agonists. Platelet aggregation without agonists is named spontaneous platelet aggregation (SPA). Since SPA has been suggested to be associated with various thrombotic diseases, it is essential to measure SPA and to establish a standard range of SPA values. In this study, we measured SPA in 167 healthy subjects by the LS method and attempted to clarify various factors influencing SPA, including the blood collection procedure. We also attempted to establish a tentative standard range of SPA values. SPA was quantitatively measured in terms of the maximum total LS intensity, which reflects small aggregates formed over 10 minutes (SMAX) and the area under the total LS intensity curve of small aggregates (SAUC). Since both the values of SMAX and SAUC were skewed and the log SMAX and log AUC values showed a normal distribution, the statistical analyses were performed using log SMAX and log SAUC. The log SMAX and log SAUC were significantly higher in the samples collected using a tourniquet and/or a 21 G needle, than in those collected without a tourniquet and/or with an 18 G needle. The log SAUC values were significantly lower in samples obtained with a syringe and/or 3.8% sodium citrate than in those obtained in vacuum sampling tubes and/or 3.13% or 3.14% sodium citrate. The Ht and plasma glucose concentration influenced the log SMAX values. We propose that to standardize SPA measurements, the measurements should be completed within two hours of blood sample collection and collected using the regular concentration of citrate. The standard range of SMAX values measured in samples obtained using a tourniquet and a 21 G needle was 2.0–23.99 (*10³ mV*count). The standard range of SAUC values measured under same conditions was 0.58–9.12 (*10⁶ mV*count*min). The standard range of SMAX values measured in samples obtained using a tourniquet, 21 G needle and a vacuum tube was 1.7–29.51 (*10³ mV*count). The standard range of SAUC values measured under same conditions was 0.59–9.33 (*10⁶ mV*count*min). KW - aggregation KW - laser KW - platelet KW - technology AU - Toshima, Hiroko AU - Sugihara, Hiroshi AU - Hamano, Hitoshi AU - Sato, Masashi AU - Yamamoto, Masahiro AU - Yamazaki, Satoshi AU - Yamada, Yukio AU - Taki, Masashi AU - Izumi, Shun-Ichiro AU - Hoshi, Keiko AU - Fusegawa, Yuichi AU - Satoh, Kaneo AU - Ozaki, Yukio AU - Kurihara, Satoshi PY - 2008/// UR - http://dx.doi.org/10.1080/09537100701883457 ER -