Mon, 07 Jul 2008 03:41:15 BST CiteULike: jyuh's Miyawaki http://www.citeulike.org/user/jyuh/author/Miyawaki CiteULike.org en-gb Copyright © 2004-2008 citeulike.org http://www.citeulike.org/user/jyuh/article/2354122 <i>Cell, Vol. 132, No. 3. (8 February 2008), pp. 487-498.</i><br /><br />Summary The cell-cycle transition from G1 to S phase has been difficult to visualize. We have harnessed antiphase oscillating proteins that mark cell-cycle transitions in order to develop genetically encoded fluorescent probes for this purpose. These probes effectively label individual G1 phase nuclei red and those in S/G2/M phases green. We were able to generate cultured cells and transgenic mice constitutively expressing the cell-cycle probes, in which every cell nucleus exhibits either red or green fluorescence. We performed time-lapse imaging to explore the spatiotemporal patterns of cell-cycle dynamics during the epithelial-mesenchymal transition of cultured cells, the migration and differentiation of neural progenitors in brain slices, and the development of tumors across blood vessels in live mice. These mice and cell lines will serve as model systems permitting unprecedented spatial and temporal resolution to help us better understand how the cell cycle is coordinated with various biological events. Visualizing Spatiotemporal Dynamics of Multicellular Cell-Cycle Progression Asako Sakaue-Sawano Hiroshi Kurokawa Toshifumi Morimura Aki Hanyu Hiroshi Hama Hatsuki Osawa Saori Kashiwagi Kiyoko Fukami Takaki Miyata Hiroyuki Miyoshi Takeshi Imamura Masaharu Ogawa Hisao Masai Atsushi Miyawaki doi:10.1016/j.cell.2007.12.033 Cell, Vol. 132, No. 3. (8 February 2008), pp. 487-498. 2008-02-08T16:32:00-00:00 2008 Cell 132 3 487 498 no-tag http://www.citeulike.org/user/jyuh/article/2607937 <i>Nature Methods, Vol. 5, No. 4. (16 March 2008), pp. 339-345.</i> Direct measurement of protein dynamics inside cells using a rationally designed photoconvertible protein Tomoki Matsuda Atsushi Miyawaki Takeharu Nagai doi:10.1038/nmeth.1193 Nature Methods, Vol. 5, No. 4. (16 March 2008), pp. 339-345. 2008-03-28T16:35:46-00:00 2008 Nature Methods 1548-7091 5 4 339 345 Nature Publishing Group no-tag http://www.citeulike.org/user/jyuh/article/2231490 <i>Diabetic Medicine, Vol. 25, No. 1. (January 2008), pp. 19-26.</i> The association of genetic variants in Kruppel-like factor 11 and Type 2 diabetes in the Japanese population Tanahashi Shinohara Keshavarz Yamaguchi Miyawaki Kunika Moritani Nakamura Yoshikawa Shiota Inoue Itakura doi:10.1111/j.1464-5491.2007.02315.x Diabetic Medicine, Vol. 25, No. 1. (January 2008), pp. 19-26. 2008-01-14T18:36:58-00:00 2008 Diabetic Medicine 0742-3071 25 1 19 26 Blackwell Publishing no-tag