Design of an Optical Switch for Studying Conformational Dynamics in Individual Molecules of GroEL

by: Gabriel A Frank, Yakov Kipnis, Elena Smolensky, Shirley S Daube, Amnon Horovitz, Gilad Haran

Bioconjugate Chem. (24 June 2008)

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DOI, American Chem. Soc. Publications

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摘要

Abstract: We describe the design of an optical switch in the chaperonin GroEL that is opened and closed by its ATP- and cochaperonin GroES-driven conformational changes. The switch, based on a fluorophore and a quencher, is engineered into the single-ring variant of the chaperone, and shows dramatic modulation of its fluorescent intensity in response to the transition of the protein between its allosteric states. It, therefore, forms a sensitive probe for the dynamics of the allosteric transitions of this machine, both in the bulk and in single molecules.

@article{citeulike:2923942, abstract = {Abstract: We describe the design of an optical switch in the chaperonin GroEL that is opened and closed by its ATP- and cochaperonin GroES-driven conformational changes. The switch, based on a fluorophore and a quencher, is engineered into the single-ring variant of the chaperone, and shows dramatic modulation of its fluorescent intensity in response to the transition of the protein between its allosteric states. It, therefore, forms a sensitive probe for the dynamics of the allosteric transitions of this machine, both in the bulk and in single molecules.}, address = {Departments of Chemical Physics, Chemical Research Support, and Structural Biology, Weizmann Institute of Science, Rehovot 76100, Israel}, author = {Frank, Gabriel A. and Kipnis, Yakov and Smolensky, Elena and Daube, Shirley S. and Horovitz, Amnon and Haran, Gilad }, citeulike-article-id = {2923942}, doi = {http://dx.doi.org/10.1021/bc800118j}, journal = {Bioconjugate Chem.}, keywords = {proteins, sms}, month = {June}, posted-at = {2008-06-24 17:01:52}, priority = {2}, title = {Design of an Optical Switch for Studying Conformational Dynamics in Individual Molecules of GroEL}, url = {http://dx.doi.org/10.1021/bc800118j}, year = {2008} }

RIS record

TY - JOUR ID - citeulike:2923942 L3 - citeulike-article-id:2923942 TI - Design of an Optical Switch for Studying Conformational Dynamics in Individual Molecules of GroEL JF - Bioconjugate Chem. AD - Departments of Chemical Physics, Chemical Research Support, and Structural Biology, Weizmann Institute of Science, Rehovot 76100, Israel N2 - Abstract: We describe the design of an optical switch in the chaperonin GroEL that is opened and closed by its ATP- and cochaperonin GroES-driven conformational changes. The switch, based on a fluorophore and a quencher, is engineered into the single-ring variant of the chaperone, and shows dramatic modulation of its fluorescent intensity in response to the transition of the protein between its allosteric states. It, therefore, forms a sensitive probe for the dynamics of the allosteric transitions of this machine, both in the bulk and in single molecules. KW - proteins KW - sms AU - Frank, Gabriel AU - Kipnis, Yakov AU - Smolensky, Elena AU - Daube, Shirley AU - Horovitz, Amnon AU - Haran, Gilad PY - 2008/06/24/ UR - http://dx.doi.org/10.1021/bc800118j ER -

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