A Photoactivatable Push−Pull Fluorophore for Single-Molecule Imaging in Live Cells

@article{citeulike:2923865, abstract = {Abstract: We have reengineered a red-emitting dicyanomethylenedihydrofuran pushpull fluorophore so that it is dark until photoactivated with a short burst of low-intensity violet light. Photoactivation of the dark fluorogen leads to conversion of an azide to an amine, which shifts the absorption to long wavelengths. After photoactivation, the fluorophore is bright and photostable enough to be imaged on the single-molecule level in living cells. This proof-of-principle demonstration provides a new class of bright photoactivatable fluorophores, as are needed for super-resolution imaging schemes that require active control of single molecule emission.}, address = {Department of Chemistry, Stanford University, Stanford, California 94305-5080, and Department of Chemistry, Kent State University, Kent, Ohio 44240}, author = {Lord, Samuel J. and Conley, Nicholas R. and Lee, Hsiao-Lu D. and Samuel, Reichel and Liu, Na and Twieg, Robert J. and Moerner, W. E. }, citeulike-article-id = {2923865}, doi = {http://dx.doi.org/10.1021/ja802883k}, journal = {J. Am. Chem. Soc.}, keywords = {click-chemistry, dcdhfs, fluorogenic, fluorophores, my-papers, photoactivatable, photochemistry}, month = {June}, posted-at = {2008-06-24 16:08:03}, priority = {0}, title = {A Photoactivatable Push\&\#x2212;Pull Fluorophore for Single-Molecule Imaging in Live Cells}, url = {http://dx.doi.org/10.1021/ja802883k}, year = {2008} }

TY - JOUR ID - citeulike:2923865 L3 - citeulike-article-id:2923865 TI - A Photoactivatable Push−Pull Fluorophore for Single-Molecule Imaging in Live Cells JF - J. Am. Chem. Soc. AD - Department of Chemistry, Stanford University, Stanford, California 94305-5080, and Department of Chemistry, Kent State University, Kent, Ohio 44240 N2 - Abstract: We have reengineered a red-emitting dicyanomethylenedihydrofuran pushpull fluorophore so that it is dark until photoactivated with a short burst of low-intensity violet light. Photoactivation of the dark fluorogen leads to conversion of an azide to an amine, which shifts the absorption to long wavelengths. After photoactivation, the fluorophore is bright and photostable enough to be imaged on the single-molecule level in living cells. This proof-of-principle demonstration provides a new class of bright photoactivatable fluorophores, as are needed for super-resolution imaging schemes that require active control of single molecule emission. KW - click-chemistry KW - dcdhfs KW - fluorogenic KW - fluorophores KW - my-papers KW - photoactivatable KW - photochemistry AU - Lord, Samuel AU - Conley, Nicholas AU - Lee, Hsiao-Lu AU - Samuel, Reichel AU - Liu, Na AU - Twieg, Robert AU - Moerner, WE PY - 2008/06/24/ UR - http://dx.doi.org/10.1021/ja802883k ER -